產(chǎn)品編號(hào) | bs-12561R |
英文名稱 | Rabbit Anti-FLRT2 antibody |
中文名稱 | 富含亮氨酸跨膜纖連蛋白2抗體 |
別 名 | Fibronectin leucine rich transmembrane protein 2; Fibronectin-like domain-containing leucine-rich transmembrane protein 2; KIAA0405; Leucine rich repeat transmembrane protein; Leucine rich repeat transmembrane protein FLRT2; FLRT2_HUMAN. |
研究領(lǐng)域 | 細(xì)胞生物 發(fā)育生物學(xué) 干細(xì)胞 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | Mouse,Rat (predicted: Human,Pig,Sheep,Cow,Chicken,Dog,Horse) |
產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 70kDa |
細(xì)胞定位 | 細(xì)胞膜 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human FLRT2: 201-300/660 <Extracellular> |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
FLRT2 is a 660 amino acid single-pass type I membrane protein that is expressed in pancreas, skeletal muscle, brain and heart. Comprised of one fibronectin type-III domain and ten LRR (leucine-rich) repeats, FLRT2 may play a role in cell adhesion and/or receptor signaling. It is suggested that FLRT2 is involved in mediating events such as NCC (neural crest cell) migration, chondrogenesis and epithelial-mesenchymal interactions during craniofacial development. Function: May have a function in cell adhesion and/or receptor signaling. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Expressed in pancreas, skeletal muscle, brain, and heart. Post-translational modifications: N-glycosylated. Similarity: Contains 1 fibronectin type-III domain. Contains 10 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain. SWISS: O43155 Gene ID: 23768 Database links: Entrez Gene: 23768 Human Entrez Gene: 399558 Mouse Omim: 604807 Human SwissProt: O43155 Human Unigene: 533710 Human Unigene: 341948 Mouse |
產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FLRT2) Polyclonal Antibody, Unconjugated (bs-12561R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-FLRT2 Polyclonal Antibody, Unconjugated(bs-12561R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
|