| 產(chǎn)品編號 | bs-12073R |
| 英文名稱 | Rabbit Anti-P2Y8 antibody |
| 中文名稱 | G蛋白偶聯(lián)嘌呤受體p2y8抗體 |
| 別 名 | P2RY8; P2RY8_HUMAN; P2Y purinoceptor 8; P2Y8; Purinergic receptor P2Y G protein coupled 8. |
| 研究領域 | 神經(jīng)生物學 通道蛋白 細胞膜受體 G蛋白偶聯(lián)受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=2ug/Test,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 41kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human P2Y8: 131-230/359 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
G protein-coupled receptors (GPRs), also known as seven transmembrane receptors, heptahelical receptors or 7TM receptors, comprise a superfamily of proteins that play a role in many different stimulus-response pathways. G-protein coupled receptors translate extracellular signals into intracellular signals (G-protein activation) and they respond to a variety of signaling molecules, such as hormones and neurotransmitters. P2RY8 (purinergic receptor P2Y, G-protein coupled, 8), also known as P2Y8, is a 359 amino acid multi-pass membrane protein that localizes to the cell membrane and belongs to the G protein-coupled receptor family. Expressed at low levels in lung, heart and kidney, P2RY8 may function as a receptor for purines that are coupled to G proteins and may also play a role in mental retardation. Function: Probable receptor for purines coupled to G-proteins. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Barely detectable in normal blood leukocytes. Weaker expression was seen in heart, kidney and lung. Not detected in brain. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: Q86VZ1 Gene ID: 286530 Database links: Entrez Gene: 286530 Human Omim: 300525 Human SwissProt: Q86VZ1 Human Unigene: 111377 Human |
| 產(chǎn)品圖片 |
Sample:
Raji Cell (Human) Lysate at 30 ug
Primary: Anti- P2Y8 (bs-12073R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 66 kD
Observed band size: 50 kD
Blank control: HL60.
Primary Antibody (green line): Rabbit Anti-P2Y8 antibody (bs-12073R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (blue line): Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: Raji.
Primary Antibody (green line): Rabbit Anti-P2Y8 antibody (bs-12073R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
