| 產(chǎn)品編號 | bs-12015R |
| 英文名稱 | Rabbit Anti-GPR18 antibody |
| 中文名稱 | G蛋白偶聯(lián)受體18抗體 |
| 別 名 | G protein coupled receptor 18; G-protein coupled receptor 18GPCRW; GPR 18; GPR18; N arachidonyl glycine receptor; NAGly receptor; GPR18_HUMAN; GPCR18. |
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Specific References (1) | bs-12015R has been referenced in 1 publications.
[IF=5.858] Matthieu Bardin. et al. The resolvin D2 – GPR18 axis is expressed in human coronary atherosclerosis and transduces atheroprotection in apolipoprotein E deficient mice. BIOCHEM PHARMACOL. 2022 Jul;201:115075 IHC ; Human.
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| 研究領(lǐng)域 | 細胞生物 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 細胞膜受體 G蛋白偶聯(lián)受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Rabbit,Pig,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=2ug/Test,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 38kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human G protein coupled receptor 18: 131-230/331 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
G protein-coupled receptors (GPRs), also known as seven transmembrane receptors, heptahelical receptors or 7TM receptors, comprise a superfamily of proteins that play a role in many different stimulus-response pathways. G protein coupled receptors translate extracellular signals into intracellular signals (G protein activation) and they respond to a variety of signaling molecules, such as hormones and neurotransmitters. GPR18 (G protein-coupled receptor 18), also known as GPCRW, is a 331 amino acid multi-pass membrane protein that belongs to the G-protein coupled receptor family. Expressed abundantly in spleen and testis, GPR18 functions as a receptor for N-arachidonyl glycine and is thought to contribute to the regulation of the immune system. GPR18 activity is mediated by G proteins that specifically inhibit adenylyl cyclase. Function: G protein coupled receptors (GPCRs, or GPRs) contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. GPCR GPR18 is a member of this family and has been reported to be expressed at high levels in testis and spleen and at lower levels in other tissues associated with endocrine and immunologic/hematologic functions. It is thought to be a receptor for N arachidonyl glycine. It may contribute to regulation of the immune system. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Most abundant in testis and spleen. Highly expressed in CD4 and CD8-positive T-cells as well as CD19-positive B-cells. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: Q14330 Gene ID: 2841 Database links: Entrez Gene: 2841 Human Entrez Gene: 110168 Mouse Omim: 602042 Human SwissProt: Q14330 Human SwissProt: Q8K1Z6 Mouse Unigene: 741589 Human Unigene: 37405 Mouse Unigene: 205907 Rat |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPR18) Polyclonal Antibody, Unconjugated (bs-12015R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
