| 產(chǎn)品編號 | bs-11966R |
| 英文名稱 | Rabbit Anti-SPOCK2 antibody |
| 中文名稱 | 睪丸蛋白聚糖2抗體 |
| 別 名 | Kazal-like domains proteoglycan 2; CWCV; FLJ97039; KIAA0275; SPARC/osteonectin; Sparc/osteonectin cwcv and kazal like domains proteoglycan (testican) 2; Sparc/osteonectin cwcv and kazal like domains proteoglycan 2; SPOCK 2; Spock2; Testican 2; Testican-2; Testican2; TICN 2; TICN2; TICN2_HUMAN. |
 |
Specific References (1) | bs-11966R has been referenced in 1 publications.
[IF=17.694] Xu An. et al. Rewired m6A epitranscriptomic networks link mutant p53 to neoplastic transformation. NAT COMMUN. 2023 Mar;14(1):1-19 IF ; Mouse.
|
| 研究領(lǐng)域 | 細胞生物 神經(jīng)生物學 細胞骨架 細胞外基質(zhì) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 45kDa |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SPOCK2/Testican 2: 61-160/424 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a protein which binds with glycosaminoglycans to form part of the extracellular matrix. The protein contains thyroglobulin type-1, follistatin-like, and calcium-binding domains, and has glycosaminoglycan attachment sites in the acidic C-terminal region. Three alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Oct 2011] Function: May participate in diverse steps of neurogenesis. Binds calcium. Subcellular Location: Secreted. Tissue Specificity: Highly expressed in brain. Also found in lung and testis. Post-translational modifications: Contains chondroitin sulfate and heparan sulfate O-linked oligosaccharides. Similarity: Contains 1 Kazal-like domain. Contains 1 thyroglobulin type-1 domain. SWISS: Q92563 Gene ID: 9806 Database links: Entrez Gene: 9806 Human Omim: 607988 Human SwissProt: Q92563 Human Unigene: 523009 Human |
| 產(chǎn)品圖片 |
Sample: Hela Cell (Human) Lysate at 40 ug
Primary: Anti-SPOCK2 (bs-11966R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPOCK2) Polyclonal Antibody, Unconjugated (bs-11966R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPOCK2) Polyclonal Antibody, Unconjugated (bs-11966R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
