| 產(chǎn)品編號 | bs-11895R |
| 英文名稱 | Rabbit Anti-NELL2 antibody |
| 中文名稱 | 蛋白激酶C結(jié)合蛋白NELL2抗體 |
| 別 名 | NRP2; A330108N19Rik; Mel91; MEL91 protein; NEL like 2 (chicken); NEL-like protein 2; Nel-related protein 2; NELL2; NELL2_HUMAN; NRP2; Protein kinase C-binding protein NELL2; R75516. |
| 研究領(lǐng)域 | 細(xì)胞生物 神經(jīng)生物學(xué) 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 89kDa |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NELL2/NRP2: 201-300/816 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a glycoprotein containing several von Willebrand factor C domains and epidermal growth factor (EGF)-like domains. The encoded protein acts as a homotrimer and is found in the cytoplasm. Several variants encoding a few different isoforms exist, and at least one isoform appears to be a secreted protein. Studies in mouse suggest that this protein plays a role in neural cell growth and differentiation as well as in oncogenesis. [provided by RefSeq, Feb 2009] Subunit: Homotrimer. Binds to PKC beta-1 Subcellular Location: Secreted. Similarity: Contains 6 EGF-like domains. Contains 1 TSP N-terminal (TSPN) domain. Contains 5 VWFC domains. SWISS: Q99435 Gene ID: 4753 Database links: Entrez Gene: 4753 Human Entrez Gene: 54003 Mouse Omim: 602320 Human SwissProt: Q99435 Human SwissProt: Q61220 Mouse Unigene: 505326 Human Unigene: 3959 Mouse |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse Placenta tissue lysates
Lane 3: Rat Testis tissue lysates
Lane 4: Rat Placenta tissue lysates
Primary: Anti-NELL2 (bs-11895R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 89 kDa
Observed band size: 135 kDa
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NELL2) Polyclonal Antibody, Unconjugated (bs-11895R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NELL2) Polyclonal Antibody, Unconjugated (bs-11895R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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