| 產(chǎn)品編號(hào) | bs-11845R |
| 英文名稱 | Rabbit Anti-GMF beta antibody |
| 中文名稱 | 膠漿成熟因子β抗體 |
| 別 名 | Glia maturation factor beta; GMF. GMF-beta; GMFB; GMFB_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 神經(jīng)生物學(xué) 生長因子和激素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human,Rabbit,Pig,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 17kDa |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GMF beta: 21-110/142 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Glia maturation factor β (GMF-β) belongs to the GMF subfamily of the larger actin-binding protein ADF family. This protein, which is phosphorylated following phorbol ester stimulation, is important for the nervous system. It causes brain cell differentiation, stimulates neural regeneration and inhibits tumor cell proliferation. Overexpression of GMF in astrocytes has been shown to enhance brain-derived neurotrohic factor (BDNF) production. GMF expression is increased by exercise, and the protein is crucial for exercise-induction of BDNF. Through BDNF production, GMF appears to play a role in neuroprotection. In thymoma, T-cell development is maintained by GMF-β being produced by the tumor cells. Function: This protein causes differentiation of brain cells, stimulation of neural regeneration, and inhibition of proliferation of tumor cells. Post-translational modifications: Phosphorylated; stimulated by phorbol ester. Similarity: Belongs to the actin-binding proteins ADF family. GMF subfamily. Contains 1 ADF-H domain. SWISS: P60983 Gene ID: 2764 Database links: Entrez Gene: 2764 Human Omim: 601713 Human SwissProt: P60983 Human Unigene: 151413 Human |
| 產(chǎn)品圖片 |
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- GMF beta (bs-11845R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17 kD
Observed band size: 19 kD
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GMF beta) Polyclonal Antibody, Unconjugated (bs-11845R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GMF beta) Polyclonal Antibody, Unconjugated (bs-11845R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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