| 產(chǎn)品編號(hào) | bs-11184R |
| 英文名稱 | Rabbit Anti-MOBP antibody |
| 中文名稱 | 少突膠質(zhì)細(xì)胞髓鞘相關(guān)蛋白抗體 |
| 別 名 | MGC87379; Myelin associated oligodendrocyte basic protein; MOBP_HUMAN. |
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Specific References (1) | bs-11184R has been referenced in 1 publications.
[IF=2.161] Kon T et al. Immunoreactivity of myelin‐associated oligodendrocytic basic protein in Lewy bodies. ? Neuropathology. 2019. WB,IHC-P&IHF ; Human,mouse&rat.
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| 研究領(lǐng)域 | 細(xì)胞生物 神經(jīng)生物學(xué) 細(xì)胞類(lèi)型標(biāo)志物 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 21kDa |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MOBP: 7-90/183 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The gene encoding MOBP a member of the central nervous system myelin-constituting proteins, maps to chromosome 3p22.2. MOBP has many splice variants that share a 68 amino acid N-terminal domain. MOBP-71, MOBP-81A, MOBP-99, and MOBP-169 are MOBP splice variants that contain exon 8b, which is similar to myelin basic protein (MBP) mRNA RTS, however MOBP-69, MOBP-81B, and MOBP-170 lack this exon. The splice variants that contain exon 8b are expressed in myelin, while those lacking exon 8b are retained in the oligodendrocyte soma. Exon 8b-containing variants are directed to sites of myelin sheath assembly by exon 8b, where they play a structural role in myelin formation. Splice variants lacking exon 8b likely play a cellular and/or regulatory role. MOBP is implicated in multiple sclerosis (MS), a human demyelinating disease, and in allergic encephalomyelitis in rodents. Function: MOBP (Myelin-associated oligodendrocytic basic protein) is abundantly expressed specifically in oligodendrocytes and is the third most abundant protein in CNS myelin. MOBP is present in the major dense line of CNS myelin suggesting a role in the compaction or stabilization of myelin. Subcellular Location: Cytoplasm, perinuclear region. Note=Present in the major dense line of CNS myelin (By similarity). SWISS: Q13875 Gene ID: 4336 Database links: Entrez Gene: 4336 Human Entrez Gene: 17433 Mouse Omim: 600948 Human SwissProt: Q13875 Human SwissProt: Q9D2P8 Mouse Unigene: 121333 Human |
| 產(chǎn)品圖片 |
Positive sample：Paraformaldehyde-fixed, paraffin embedded (rat brian);
Negative sample：Paraformaldehyde-fixed, paraffin embedded (rat liver);
Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MOBP) Polyclonal Antibody, Unconjugated (bs-11184R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MOBP) Polyclonal Antibody, Unconjugated (bs-11184R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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