| 產(chǎn)品編號 | bs-11176R |
| 英文名稱 | Rabbit Anti-Pregnancy zone protein antibody |
| 中文名稱 | 妊娠區(qū)帶蛋白PZP抗體 |
| 別 名 | A1m antibody; C3 and PZP like alpha-2 macroglobulin domain containing protein 6; Complement component 3 and pregnancy zone protein like alpha 2 macroglobulin domain containing protein 6; CPAMD6; PZP; PZP_HUMAN. |
| 研究領域 | 神經(jīng)生物學 細胞粘附分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Cow,Dog,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 161kDa |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Pregnancy zone protein: 951-1050/1482 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Pregnancy Zone protein is a 1,482 amino acid secreted protein that belongs to the protease inhibitor I39 family and exists as multiple alternatively spliced isoforms. Expressed predominately in plasma and in late-pregnancy sera, Pregnancy Zone protein functions as a disulfide-linked homotetramer that is able to trap and inhibit proteinases, thus playing a role in the regulation of protein splitting and small peptide formation. The gene encoding Pregnancy Zone protein maps to human chromosome 12, which encodes over 1,100 genes and comprises approximately 4.5% of the human genome. Chromosome 12 is associated with a variety of diseases and afflictions, including hypochondrogenesis, achondrogenesis, Kniest dysplasia, Noonan syndrome and Trisomy 12p, which causes facial developmental defects and seizure disorders. Function: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. Subunit: Homotetramer, which consists of two pairs of disulfide-linked chains. Subcellular Location: Secreted. Similarity: Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family. SWISS: P20742 Gene ID: 5858 Database links: Entrez Gene: 5858 Human Omim: 176420 Human SwissProt: P20742 Human |
| 產(chǎn)品圖片 |
Sample:
Plasma (Mouse) Lysate at 40 ug
Serum (Mouse) Lysate at 40 ug
Primary: Anti-Pregnancy zone protein (bs-11176R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 161 kD
Observed band size: 161 kD
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
