| 產(chǎn)品編號(hào) | bs-9865R |
| 英文名稱(chēng) | Rabbit Anti-MYLK3 antibody |
| 中文名稱(chēng) | 肌球蛋白輕鏈激酶3抗體 |
| 別 名 | caMLCK; Cardiac MyBP C associated Ca/CaM kinase; Cardiac-MyBP-C-associated Ca/CaM kinase; MLC kinase; MLCK; MLCK2; Mylk3; MYLK3_HUMAN; Myosin light chain kinase 3; Putative myosin light chain kinase 3. |
| 研究領(lǐng)域 | 心血管 信號(hào)轉(zhuǎn)導(dǎo) 激酶和磷酸酶 細(xì)胞骨架 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Rabbit,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 98kDa |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MYLK3: 701-800/817 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The Ca2+/calmodulin-dependent protein kinases (CaM kinases) are a structurally related subfamily of serine/threonine kinases that includes CaMKI, CaMKII, CaMKIV and Myosin light chain kinases (MYLKs, also designated MLCKs). The MYLK kinases phosphorylate Myosin regulatory light chains to catalyze Myosin interaction with Actin filaments, resulting in contractile activity. MYLK3 (Myosin light chain kinase 3) is a 795 amino acid cardiac-specific protein that contains one protein kinase domain and belongs to the protein kinase superfamily. Like other MYLK kinases, MYLK3 is thought to play a role in smooth muscle contraction, specifically using magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of Myosin light chain proteins. Additionally, MYLK3 may regulate sarcomere assembly in heart tissue, possibly mediating proper heart function. Function: Kinase that phosphorylates MYL2 in vitro. Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility (By similarity). Subcellular Location: Cytoplasm. Tissue Specificity: May play a role in smooth muscle contraction. Post-translational modifications: Phosphorylated on serine residues (By similarity). Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Contains 1 protein kinase domain. SWISS: Q32MK0 Gene ID: 91807 Database links: Entrez Gene: 91807 Human Omim: 612147 Human SwissProt: Q32MK0 Human Unigene: 130465 Human |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with MYLK3 polyclonal antibody, unconjugated (bs-9865R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Sample:
Bone (Mouse) Lysate at 40 ug
Primary: Anti- MYLK3 (bs-9865R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 98 kD
Observed band size: 100 kD
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