| 產(chǎn)品編號(hào) | bs-9396R |
| 英文名稱(chēng) | Rabbit Anti-phospho-CD130/gp130 (Ser782) antibody |
| 中文名稱(chēng) | 磷酸化gp130抗體 |
| 別 名 | gp130 (phospho S782); gp130 (phospho Ser782); p-gp130 (Ser782); Interleukin-6 receptor subunit beta; IL-6R-beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; gp130; CDw130; Oncostatin-M receptor subunit alpha; CD_antigen; CD130; GP130 RAPS; gp130 transducer chain; GP130-RAPS; IL6 ST; IL6R-beta; IL6ST.IL6R-beta; IL6RB_HUMAN; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6RB. |
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Specific References (1) | bs-9396R has been referenced in 1 publications.
[IF=7.76] Honke, Nora, et al. "The p38‐Mediated Rapid Down‐Regulation of Cell Surface gp130 Expression Impairs Interleukin‐6 Signaling in the Synovial Fluid of Juvenile Idiopathic Arthritis Patients." Arthritis & Rheumatology 66.2 (2014): 470-478. FCM ; Human.
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| 產(chǎn)品類(lèi)型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞膜受體 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human,Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 99kDa |
| 細(xì)胞定位 | 細(xì)胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human gp130 around the phosphorylation site of Ser 782: SE(p-S)TQ |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
CD130 is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggested a critical role of the gene encoding this protein in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. Function: Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development. The type I OSM receptor is capable of transducing OSM-specific signaling events. Subunit: Interacts with INPP5D/SHIP1. Forms heterodimers composed of LIPR and IL6ST (type I OSM receptor). Also forms heterodimers composed of OSMR and IL6ST (type II OSM receptor). Homodimer. The homodimer binds two molecules of herpes virus IL6. Component of a hexamer of two molecules each of IL6, IL6R and IL6ST. Interacts with HCK. Subcellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. Tissue Specificity: Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells. Post-translational modifications: Phosphorylation of Ser-782 down-regulates cell surface expression. Heavily N-glycosylated. Similarity: Belongs to the type I cytokine receptor family. Type 2 subfamily. Contains 5 fibronectin type-III domains. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. SWISS: P40189 Gene ID: 3572 Database links: Entrez Gene: 3572 Human Entrez Gene: 16195 Mouse Omim: 600694 Human SwissProt: P40189 Human SwissProt: Q00560 Mouse Unigene: 532082 Human Unigene: 706627 Human Unigene: 4364 Mouse |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Liver (Mouse) Lysate at 40 ug
Lane 2: Liver (Rat) Lysate at 40 ug
Lane 3: Lung (Rat) Lysate at 40 ug
Primary: Anti-phospho-CD130/gp130 (Ser782) (bs-9396R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 140 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-gp130 (Ser782) Polyclonal Antibody, Unconjugated(bs-9396R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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