| 產(chǎn)品編號 | bs-6998R |
| 英文名稱 | Rabbit Anti-ERR-alpha antibody |
| 中文名稱 | 雌激素受體相關(guān)蛋白α抗體 |
| 別 名 | Estrogen Related Receptor alpha; ERR-α; ERR α; ERRα; ERR alpha; Err 1; ERR a; ERR alpha; ERR-alpha; Err1; ERR1_HUMAN; ERRa; ERRalpha; ESRL 1; ESRL1; ESRR A; Esrra; Estrogen receptor like 1; Estrogen receptor related 1; Estrogen receptor-like 1; Estrogen related receptor alpha; Estrogen-related receptor alpha; Estrra; Nr3b1; Nuclear receptor subfamily 3 group B member 1; Steroid hormone receptor ERR1. |
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Specific References (2) | bs-6998R has been referenced in 2 publications.
[IF=3.159] Wan Boyang. et al. Zearalenone promotes follicle development through activating SIRT1/PGC-1α signaling pathway in the ovaries of weaned gilts. J Anim Sci. 2022 Feb;: WB ; Pig(Gilt).
[IF=2.447] Panpan Dong. et al. Roles of ERRα and TGF?β signaling in stemness enhancement induced by 1?μM bisphenol?A exposure via human neural stem cells. Exp Ther Med. 2022 Feb;23(2):1-10 WB ; Human.
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| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 染色質(zhì)和核信號 信號轉(zhuǎn)導(dǎo) 生長因子和激素 結(jié)合蛋白 細(xì)胞骨架 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Pig,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 47kDa |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Estrogen Related Receptor alpha: 101-200/423 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism. Subunit: Binds DNA as a monomer or a homodimer. Interacts (via the AF2 domain) with coactivator PPARGC1A (via the L3 motif); the interaction greatly enhances transriptional activity of genes involved in energy metabolism. Interacts with PIAS4; the interaction enhances sumoylation. Subcellular Location: Nucleus. Similarity: Belongs to the nuclear hormone receptor family. NR3 subfamily. Contains 1 nuclear receptor DNA-binding domain. SWISS: P11474 Gene ID: 2101 Database links: Entrez Gene: 2101 Human Entrez Gene: 26379 Mouse Omim: 601998 Human SwissProt: P11474 Human SwissProt: O08580 Mouse Unigene: 110849 Human Unigene: 386776 Mouse Unigene: 130171 Rat |
| 產(chǎn)品圖片 |
Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-ERR-alpha (bs-6998R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 47 kD
Sample:
Lovp(Human) Cell Lysate at 30 ug
Primary: Anti- ERR-alpha (bs-6998R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 47 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ERR-alpha) Polyclonal Antibody, Unconjugated (bs-6998R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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