| 產(chǎn)品編號 | bs-9183R |
| 英文名稱 | Rabbit Anti-VGLL2 antibody |
| 中文名稱 | 轉(zhuǎn)錄輔助因子退變樣蛋白2抗體 |
| 別 名 | Protein VITO1; Transcription cofactor vestigial like 2; Transcription cofactor vestigial like protein 2; Transcription cofactor vestigial-like protein 2; Vestigial like 2; Vestigial like 2 (Drosophila); Vgl-2; VGL2; VGLL 2; Vgll2; VGLL2_HUMAN; VITO1. |
| 研究領域 | 腫瘤 細胞生物 免疫學 信號轉(zhuǎn)導 干細胞 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Pig,Cow,Dog) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 33kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human VGLL2: 101-200/317 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Vgl-2, also known as VITO-1, is a 317 amino acid protein that contains a domain through which it interacts with TEF-1, a protein that plays a role in controlling the expression of numerous genes. Specific to skeletal muscle, Vgl-2 is expressed highly in adult fast muscle and is expressed at lower levels in adult slow muscle and fetal skeletal muscle. During muscle differentiation, Vgl-2 mRNA levels increase and Vgl-2 translocates from the cytoplasm to the nucleus. Overexpression of Vgl-2 in MYOD-transfected 10T1/2 mouse embryonic fibroblasts increases expression of myosin heavy chain (MHC), which is a marker of terminal muscle differentiation. This evidence suggests that Vgl-2 is essential for muscle gene expression. There are two isoforms of Vgl-2 that are produced as a result of alternative splicing events.
Function: May act as a specific coactivator for the mammalian TEFs. May play a role in the development of skeletal muscles. Subunit: Interacts with TEFs. Binds to TEAD1/TEF1. Subcellular Location: Nucleus. Tissue Specificity: Skeletal muscle. Similarity: Belongs to the vestigial family. SWISS: Q8N8G2 Gene ID: 245806 Database links: Entrez Gene: 245806 Human Entrez Gene: 215031 Mouse Omim: 609979 Human SwissProt: Q8N8G2 Human SwissProt: Q8BGW8 Mouse Unigene: 99324 Human Unigene: 87237 Mouse |
| 產(chǎn)品圖片 |
Sample: Muscle (Mouse) Lysate at 40 ug
Primary: Anti-VGLL2 (bs-9183R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 35 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
