產(chǎn)品編號 | bs-6467R |
英文名稱 | Rabbit Anti-Dlx1 antibody |
中文名稱 | 同源轉(zhuǎn)錄因子DLX1抗體 |
別 名 | DII B; Distal less homeo box 1; Distal less homeobox 1; Distal-less homeobox gene 1a; Dlx 1; Dlx; Dlx1; Dlx1 distal-less homeobox 1; DLX1_HUMAN; Homeo box protein DLX1; Homeobox protein DLX 1; Homeobox protein DLX-1; Homeobox protein Dlx1a; Homeodomain transcription factor DLX1; RP23-419H3.3. |
研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 干細(xì)胞 表觀遺傳學(xué) |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | Human (predicted: Mouse,Rat,Rabbit,Pig,Cow,Chicken,Dog,Horse) |
產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=1ug/test,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 28kDa |
細(xì)胞定位 | 細(xì)胞核 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human Dlx1: 101-200/255 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
Likely to play a regulatory role in the development of the ventral forebrain. May play a role in craniofacial patterning and morphogenesis and may be involved in the early development of diencephalic subdivisions. Function: Likely to play a regulatory role in the development of the ventral forebrain. May play a role in craniofacial patterning and morphogenesis and may be involved in the early development of diencephalic subdivisions (By similarity). Subcellular Location: Nucleus. Similarity: Belongs to the distal-less homeobox family. Contains 1 homeobox DNA-binding domain. SWISS: P56177 Gene ID: 1745 Database links: Entrez Gene: 1745 Human Entrez Gene: 13390 Mouse Omim: 600029 Human SwissProt: P56177 Human SwissProt: Q64317 Mouse Unigene: 407015 Human Unigene: 4543 Mouse Unigene: 475101 Mouse Unigene: 59152 Rat |
產(chǎn)品圖片 |
Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-Dlx1 antibody (bs-6467R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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