| 產(chǎn)品編號 | bs-8070R |
| 英文名稱 | Rabbit Anti-ADCK4 antibody |
| 中文名稱 | ADCK4蛋白抗體 |
| 別 名 | aarF domain containing protein kinase 4; Uncharacterized aarF domain containing protein kinase 4; ADCK4_HUMAN. |
| 研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Cow,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:200-800,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 60kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ADCK4: 65-170/544 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
ADCK4, AarF domain containing kinase 5, is a member of group i collagen (fibrillar forming collagen). The function of this protein is not yet clear. It is not known if it has protein kinase activity and what type of substrate it would phosphorylate (Ser, Thr or Tyr). There are 2 named isoforms produced by alternative splicing. Function: The function of this protein is not yet clear. It is notknown if it has protein kinase activity and what type of substrateit would phosphorylate (Ser, Thr or Tyr). Subcellular Location: Membrane; Single-pass membrane protein(Potential). Similarity: Belongs to the protein kinase superfamily. ADCKprotein kinase family. Contains 1 protein kinase domain. SWISS: Q96D53 Gene ID: 79934 Database links: Entrez Gene: 79934 Human Entrez Gene: 76889 Mouse Omim: 615567 Human SwissProt: Q96D53 Human SwissProt: Q566J8 Mouse Unigene: 130712 Human Unigene: 124728 Mouse Unigene: 11549 Rat |
| 產(chǎn)品圖片 |
Tissue/cell: HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (ADCK4) Polyclonal Antibody, Unconjugated (bs-8070R) 1:200, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ADCK4) Polyclonal Antibody, Unconjugated (bs-8070R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ADCK4) Polyclonal Antibody, Unconjugated (bs-8070R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
