| 產品編號 | bs-6916R |
| 英文名稱 | Rabbit Anti-RABX5 antibody |
| 中文名稱 | G蛋白相關RABX5抗體 |
| 別 名 | Rabex5; RAB guanine nucleotide exchange factor (GEF) 1; Rab5 GDP/GTP exchange factor; Rab5ef; Rabaptin 5 associated exchange factor for Rab5; Rabaptin-5-associated exchange factor for Rab5; Rabex 5; Rabex-5; RABGEF 1; Rabgef1; RABX 5; RABX5; RABX5_HUMAN; RAP1; Rin 2; Rin2. |
| 研究領域 | 細胞生物 信號轉導 轉錄調節(jié)因子 G蛋白偶聯(lián)受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human,Mouse,Rat,Rabbit,Pig,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 79kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RABX5: 251-350/708 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
RABGEF1 forms a complex with rabaptin-5 (RABPT5; MIM 603616) that is required for endocytic membrane fusion, and it serves as a specific guanine nucleotide exchange factor (GEF) for RAB5 (RAB5A; MIM 179512) (Horiuchi et al., 1997 [PubMed 9323142]).[supplied by OMIM, Mar 2010] Function: Rab effector protein acting as linker between gamma-adaptin, RAB4A or RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates nucleotide exchange on RAB5A. Can act as a ubiquitin ligase (By similarity). Subunit: Interacts with RGS14; the interaction is GTP-dependent (By similarity). Heterodimer with RABEP1. The heterodimer binds RAB4A and RAB5A that have been activated by GTP-binding. Interacts with RAB21, and with 100-fold lower affinity also with RAB22. Binds TSC2, GGA1, GGA2, GGA3, AP1G1 and AP1G2. Interacts with ubiquitinated EGFR Subcellular Location: Cytoplasm. Early endosome. Recycling endosome. Post-translational modifications: Monoubiquitinated. Similarity: Contains 1 A20-type zinc finger. Contains 1 VPS9 domain. SWISS: Q9UJ41 Gene ID: 27342 Database links: Entrez Gene: 27342 Human Entrez Gene: 56715 Mouse Omim: 609700 Human SwissProt: Q9UJ41 Human SwissProt: Q9JM13 Mouse Unigene: 530053 Human Unigene: 288639 Mouse Unigene: 21688 Rat |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
