產(chǎn)品編號 | bs-7914R |
英文名稱 | Rabbit Anti-MOBKL2B antibody |
中文名稱 | Mob1同源蛋白MOBKL2B抗體 |
別 名 | Mob1 homolog 2b; MOB1 Mps One Binder kinase activator like 2B (yeast); MOB1 Mps One Binder kinase activator like 2B; MOB3B; MOBKL2B; MOL2B_HUMAN; monopolar spindle 1 binding MOB1 domain containing; Mps one binder kinase activator-like 2B; Protein Mob3B. |
研究領域 | 細胞生物 信號轉(zhuǎn)導 細胞周期蛋白 激酶和磷酸酶 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Pig,Cow,Chicken,Horse) |
產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 25kDa |
細胞定位 | 細胞核 細胞漿 細胞外基質(zhì) 分泌型蛋白 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human MOBKL2B: 88-200/216 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
The protein encoded by this gene shares similarity with the yeast Mob1 protein. Yeast Mob1 binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation. This gene is located on the opposite strand as the interferon kappa precursor (IFNK) gene. [provided by RefSeq, Jul 2008].
Function: May regulate the activity of kinases (By similarity). Similarity: Belongs to the MOB1/phocein family. SWISS: Q86TA1 Gene ID: 79817 Database links: UniProtKB/Swiss-Prot: Q86TA1.2 |
產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MOBKL2B) Polyclonal Antibody, Unconjugated (bs-7914R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MOBKL2B) Polyclonal Antibody, Unconjugated (bs-7914R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關(guān)于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |