| 產(chǎn)品編號 | bs-7665R |
| 英文名稱 | Rabbit Anti-NUP50 antibody |
| 中文名稱 | 核孔蛋白50抗體 |
| 別 名 | NPAP60; NPAP60L; Nuclear pore-associated protein 60 kDa-like; nucleoporin 50; NUP50_HUMAN. |
| 研究領(lǐng)域 | 細(xì)胞生物 信號轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Rat (predicted: Human,Mouse,Rabbit,Pig,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 50kDa |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NUP50: 351-450/468 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
NUP50 is a component of the nuclear pore complex that has a direct role in nuclear protein export. NUP50 may serve as a binding site on the nuclear side of the pore complex for export receptor-cargo complexes. NUP50 interacts with multiple transport receptor proteins including p27Kip1. This interaction is required for correct intracellular transport and degradation of p27Kip1. Function: Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with multiple transport receptor proteins including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B. Subunit: Interacts with Importin alpha-2, Importin beta, Importin beta-2, NUP153, Ran binding protein 7, CDKN1B and itself (By similarity). Subcellular Location: Nucleus, nuclear pore complex. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side (By similarity). Note=Localizes to the nucleoplasmic fibrils of the nuclear pore complex (By similarity). Tissue Specificity: Ubiquitous. Highest levels in testis, peripheral blood leukocytes and fetal liver. Similarity: Contains 1 RanBD1 domain. SWISS: Q9UKX7 Gene ID: 10762 Database links: Entrez Gene: 10762 Human Entrez Gene: 18141 Mouse Omim: 604646 Human SwissProt: Q9UKX7 Human SwissProt: Q9JIH2 Mouse Unigene: 727643 Human Unigene: 28379 Mouse Unigene: 391486 Mouse Unigene: 123156 Rat Unigene: 3242 Rat |
| 產(chǎn)品圖片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NUP50 Polyclonal Antibody, Unconjugated(bs-7665R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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