| 產品編號 | bs-7647R |
| 英文名稱 | Rabbit Anti-VDAC3 antibody |
| 中文名稱 | 線粒體外膜孔蛋白3/電壓依賴陰離子通道蛋白3抗體 |
| 別 名 | hVDAC3; mVDAC3; Outer mitochondrial membrane protein porin 3; voltage dependent anion channel 3; VDAC3_HUMAN. |
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Specific References (4) | bs-7647R has been referenced in 4 publications.
[IF=6.792] Zixuan Liu. et al. Iron-bearing nanoparticles trigger human umbilical vein endothelial cells ferroptotic responses by promoting intracellular iron level. Environ Pollut. 2021 Oct;287:117345 WB ; Human.
[IF=5.638] Qi H et al. Succinylation-dependent mitochondrial translocation of PKM2 promotes cell survival in response to nutritional stress. Cell Death Dis. 2019 Feb 20;10(3):170. Co-IP ; Human.
[IF=4.101] Haixiang Ding. et al. Ailanthone suppresses the activity of human colorectal cancer cells through the STAT3 signaling pathway. Int J Mol Med. 2022 Feb;49(2):1-11 WB ; Human.
[IF=3.076] Tang Q et al. Ferroptosis is newly characterized form of neuronal cell death in response to arsenite exposure.Neurotoxicology. 2018 Jul;67:27-36. WB ; Mouse.
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| 研究領域 | 細胞生物 信號轉導 細胞凋亡 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 31kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human VDAC3: 101-200/283 |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a voltage-dependent anion channel (VDAC), and belongs to the mitochondrial porin family. VDACs are small, integral membrane proteins that traverse the outer mitochondrial membrane and conduct ATP and other small metabolites. They are known to bind several kinases of intermediary metabolism, thought to be involved in translocation of adenine nucleotides, and are hypothesized to form part of the mitochondrial permeability transition pore, which results in the release of cytochrome c at the onset of apoptotic cell death. Alternatively transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011] Function: Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules (By similarity). Subcellular Location: Mitochondrion outer membrane. Tissue Specificity: Widely expressed. Highest in testis. Similarity: Belongs to the eukaryotic mitochondrial porin family. SWISS: Q9Y277 Gene ID: 7419 Database links: Entrez Gene: 426793 Chicken Entrez Gene: 7419 Human Entrez Gene: 22335 Mouse Omim: 610029 Human SwissProt: Q9Y277 Human SwissProt: Q60931 Mouse SwissProt: Q9TT13 Rabbit Unigene: 699301 Human Unigene: 227704 Mouse Unigene: 107287 Rat |
| 產品圖片 |
Sample:
Lane 1: Mouse Kidney tissue lysates
Lane 2: Mouse Spleen tissue lysates
Lane 3: Rat Kidney tissue lysates
Lane 4: Rat Spleen tissue lysates
Primary: Anti-VDAC3 (bs-7647R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa
Sample:
Large intestine(Rat) Lysate at 40 ug
Primary: Anti- VDAC3 (bs-7647R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD
Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-VDAC3 Polyclonal Antibody, Unconjugated(bs-7647R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
