| 產(chǎn)品編號 | bs-2077R |
| 英文名稱 | Rabbit Anti-AMPD1 antibody |
| 中文名稱 | 腺苷單磷酸脫氨酶1抗體 |
| 別 名 | AMPD1; AMPD-1; AMPD1; AMPD-1; Adenosine monophosphate deaminase 1 (isoform M); Adenosine monophosphate deaminase 1; AMP deaminase 1; AMP deaminase isoform M; AMPD 1; Ampd01; MAD; MADA; Myoadenylate deaminase; RATAMPD01; AMPD1_MOUSE. |
| 研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導(dǎo) 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=2ug/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 86kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse AMP deaminase 1: 101-200/745 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
AMP deaminase (AMPD) is an allosteric enzyme that plays a critical role in energy metabolism. There are three functional isoforms of AMPD. AMPD1 is the skeletal muscle-specific isoform M located in type II muscle fibers, neuromuscular junctions and in capillaries. Defects in AMPD1 are the cause of adenosine monophosphate deaminase deficiency muscle type (AMPDDM). AMPDDM is a metabolic disorder resulting in exercise-related myopathy. It is characterized by exercise-induced muscle aches, cramps, and early fatigue. Function: AMP deaminase plays a critical role in energy metabolism. Subunit: Homotetramer Similarity: Belongs to the adenosine and AMP deaminases family. SWISS: Q3V1D3 Gene ID: 270 Database links: Entrez Gene: 270 Human Entrez Gene: 229665 Mouse Omim: 102770 Human SwissProt: P23109 Human SwissProt: Q3V1D3 Mouse Unigene: 89570 Human Unigene: 331272 Mouse Unigene: 9794 Rat |
| 產(chǎn)品圖片 |
Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-AMPD1 antibody (bs-2077R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ethanol (10min at room temperature) and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
