| 產(chǎn)品編號 | bs-7582R |
| 英文名稱 | Rabbit Anti-BCL2L15 antibody |
| 中文名稱 | BCL2樣15促凋亡蛋白抗體 |
| 別 名 | B2L15_HUMAN; Bcl-2-like protein 15; Bcl2-L-15; BCL2-like 15; Bcl2l15; Bfk; C1orf178; FLJ22588; pro-apoptotic Bcl-2 protein. |
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Specific References (1) | bs-7582R has been referenced in 1 publications.
[IF=3.759] Diqi Yanget al. BCL2L15 Depletion Inhibits Endometrial Receptivity via the STAT1 Signaling Pathway. Genes (Basel)
. 2020 Jul 17;11(7):816. WB, IF ; Goat.
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| 研究領(lǐng)域 | 細胞生物 細胞凋亡 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Rabbit,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:200-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 18kDa |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BCL2L15: 65-163/163 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
BCL2L15 is a 163 amino acid protein and novel member of the Bcl-2 family that contains both BH2 and BH3 regions, but not BH1, BH4 or a C-terminal hydrophobic membrane anchor. Like Bcl-GL, Bfk does not bind to any Bcl-2 family members, even though its BH3 motif can mediate association with prosurvival proteins. Bfk localizes to cytoplasm, but unlike Bcl-GL, Bfk does not associate with organelles. Existing as four alternatively spliced isoforms, the pro-apoptotic isoforms of Bfk may help to protect against the development of human gastrointestinal malignancy. Bfk is found at low levels in stomach, ovary, bone marrow and spleen, but Bfk is highly expressed in mammary gland during pregnancy, suggesting that Bfk may play a role in mammary development. Bfk appears to be a cytosolic protein whose gene maps to chromosome 1p13.2. Chromosome 1 is the largest human chromosome spanning about 260 million base pairs and making up 8% of the human genome. SWISS: Q5TBC7 Gene ID: 440603 Database links: UniProtKB/Swiss-Prot: Q5TBC7.1 Entrez Gene: 440603 Human SwissProt: Q5TBC7 Human Unigene: 123106 Human
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| 產(chǎn)品圖片 |
Sample:
Lane 1: Human SW480 cell lysates
Lane 2: Human MCF-7 cell lysates
Lane 3: Human A549 cell lysates
Primary: Anti-BCL2L15 (bs-7582R) at 1/200 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18kDa
Observed band size: 18 kDa
Sample:
Raji(Human) Cell Lysate at 30 ug
Primary: Anti-BCL2L15 (bs-7582R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18 kD
Observed band size: 19kD
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BCL2L15) Polyclonal Antibody, Unconjugated (bs-7582R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
