| 產(chǎn)品編號(hào) | bs-6592R |
| 英文名稱(chēng) | Rabbit Anti-HSD17B6 antibody |
| 中文名稱(chēng) | 17-β-羥脫氫酶6抗體 |
| 別 名 | 17-beta-HSD 6; 17-beta-HSD6; 17-beta-hydroxysteroid dehydrogenase type 6; 3 hydroxysteroid epimerase; 3(alpha >beta) hydroxysteroid epimerase; 3(alpha >beta) hydroxysteroid epimerasel; 3-alpha->beta-HSE; 3-alpha->beta-hydroxysteroid epimerase; H17B6_HUMAN; HSD17B6; HSE; Hydroxysteroid (17 beta) dehydrogenase 6; Hydroxysteroid (17 beta) dehydrogenase 6 homolog (mouse); Hydroxysteroid 17 beta dehydrogenase 6; NAD+ dependent 3 alpha hydroxysteroid dehydrogenase 3 hydroxysteroid epimerase; NAD+ dependent 3 alpha hydroxysteroid dehydrogenase; Oxidative 3 alpha hydroxysteroid dehydrogenase; Oxidative 3-alpha hydroxysteroid dehydrogenase; Oxidoreductase; Retinol dehydrogenase; RODH; SDR9C6;Short chain dehydrogenase/reductase family 9C, member 6. |
| 研究領(lǐng)域 | 信號(hào)轉(zhuǎn)導(dǎo) 生長(zhǎng)因子和激素 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Pig,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1ug/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 33kDa |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HSD17B6: 61-160/317 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene has both oxidoreductase and epimerase activities and is involved in androgen catabolism. The oxidoreductase activity can convert 3 alpha-adiol to dihydrotestosterone, while the epimerase activity can convert androsterone to epi-androsterone. Both reactions use NAD+ as the preferred cofactor. This gene is a member of the retinol dehydrogenase family. [provided by RefSeq, Aug 2013] Function: NAD-dependent oxidoreductase with broad substrate specificity that shows both oxidative and reductive activity (in vitro). Has 17-beta-hydroxysteroid dehydrogenase activity towards various steroids (in vitro). Converts 5-alpha-androstan-3-alpha,17-beta-diol to androsterone and estradiol to estrone (in vitro). Has 3-alpha-hydroxysteroid dehydrogenase activity towards androsterone (in vitro). Has retinol dehydrogenase activity towards all-trans-retinol (in vitro). Can convert androsterone to epi-androsterone. Androsterone is first oxidized to 5-alpha-androstane-3,17-dione and then reduced to epi-andosterone. Can act on both C-19 and C-21 3-alpha-hydroxysteroids. Subcellular Location: Microsome membrane; Peripheral membrane protein; Lumenal side. Early endosome membrane; Peripheral membrane protein; Lumenal side (Potential). Tissue Specificity: Detected in liver and prostate (at protein level). Detected in adult liver, lung, brain, placenta, prostate, adrenal gland, testis, mammary gland, spleen, spinal cord and uterus. Detected in caudate nucleus, and at lower levels in amygdala, corpus callosum, hippocampus, substantia nigra and thalamus. Detected in fetal lung, liver and brain. Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. SWISS: O14756 Gene ID: 8630 Database links: Entrez Gene: 8630 Human Entrez Gene: 27400 Mouse Omim: 606623 Human SwissProt: O14756 Human SwissProt: Q9R092 Mouse Unigene: 524513 Human Unigene: 26719 Mouse Unigene: 10857 Rat |
| 產(chǎn)品圖片 |
Molt-4 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HSD17B6 Antibody(bs-6592R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Blank control (Black line): Molt4 (Black).
Primary Antibody (green line):Rabbit Anti-HSD17B6 antibody (bs-6592R)
Dilution:3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 3μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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