| 產(chǎn)品編號(hào) | bs-6261R |
| 英文名稱 | Rabbit Anti-NEK3 antibody |
| 中文名稱 | 絲氨酸/蘇氨酸蛋白激酶Nek3抗體 |
| 別 名 | HSPK 36; HSPK36; HSPK-36; NEK3; NEK 3; NEK-3; NEK3_HUMAN; Never in mitosis A-related kinase 3; NIMA (never in mitosis gene a) related kinase 3; NimA related protein kinase 3; NimA-related protein kinase 3; Serine/threonine protein kinase Nek3; Serine/threonine-protein kinase Nek3. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Rabbit,Pig,Cow,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 58kDa |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NEK3: 165-265/506 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar2009]. Function: Protein kinase which influences neuronal morphogenesis and polarity through effects on microtubules. Regulates microtubule acetylation in neurons. Contributes to prolactin-mediated phosphorylation of PXN and VAV2. Implicated in prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells through mechanisms involving RAC1 activation and phosphorylation of PXN and VAV2. Subunit: Interacts with PXN, PRLR, VAV1 and VAV2 and this interaction is prolactin-dependent. Subcellular Location: Cytoplasm (By similarity). Cell projection, axon (By similarity). Tissue Specificity: Up-regulated in malignant versus normal breast tissue. Isoform 2 shows a high level of expression in testis, ovary and brain. Post-translational modifications: Phosphorylation at Thr-479 regulates its catalytic activity (By similarity). Similarity: Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. Contains 1 protein kinase domain. SWISS: P51956 Gene ID: 4752 Database links: Entrez Gene: 4752 Human Entrez Gene: 23954 Mouse Omim: 604044 Human SwissProt: P51956 Human SwissProt: Q9R0A5 Mouse Unigene: 409989 Human Unigene: 41413 Mouse Unigene: 45730 Rat |
| 產(chǎn)品圖片 |
Sample:
Small intestine (Mouse) Lysate at 40 ug
Large intestine (Mouse) Lysate at 40 ug
Urinary bladder (Mouse) Lysate at 40 ug
Stomach (Mouse) Lysate at 40 ug
Primary: Anti-NEK3 (bs-6261R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD
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