| 產(chǎn)品編號 | bs-6234R |
| 英文名稱 | Rabbit Anti-DOK3 antibody |
| 中文名稱 | 對接蛋白3抗體 |
| 別 名 | Docking protein 3; DOK 3; DOK Like Protein; DOKL Pending; Downstream of tyrosine kinase 3; p62 DOK Like Protein; DOK3_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 信號轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Cow) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 53kDa |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DOK3: 45-145/496 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate Abl functionDOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate Abl function.
There are 4 isoforms generated by alternative splicing. Function: DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with Subunit: On tyrosine phosphorylation, interacts with CSK and INPP5D/SHIP1 via their SH2 domains. Both Tyr-381 and Tyr-398 are required for interaction with INPP5D. Only Tyr-381 is required for interaction with CSK. Binds ABL1 through the PTB domain and in a kinase-dependent manner. Does not interact with RasGAP (By similarity). Subcellular Location: Cytoplasm (By similarity). Cell membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Tissue Specificity: Expressed in spleen. Post-translational modifications: Constitutively tyrosine-phosphorylated (By similarity). On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL1 kinase (By similarity). Similarity: Belongs to the DOK family. Type A subfamily. Contains 1 IRS-type PTB domain. Contains 1 PH domain. SWISS: Q7L591 Gene ID: 79930 Database links: Entrez Gene: 79930 Human Entrez Gene: 27261 Mouse Omim: 611435 Human SwissProt: Q7L591 Human SwissProt: Q9QZK7 Mouse Unigene: 720849 Human Unigene: 33910 Mouse Unigene: 19911 Rat |
| 產(chǎn)品圖片 |
Sample:
SP2/0(Mouse) Cell Lysate at 30 ug
Primary: Anti-DOK3 (bs-6234R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti-DOK3 (bs-6234R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
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