| 產(chǎn)品編號 | bs-5973R |
| 英文名稱 | Rabbit Anti-SIRT7 antibody |
| 中文名稱 | 沉默調(diào)節(jié)樣蛋白SirT7抗體 |
| 別 名 | NAD dependent deacetylase sirtuin 7; NAD-dependent deacetylase sirtuin-7; Silent mating type information regulation 2 homolog; Silent mating type information regulation 2 S.cerevisiae homolog 7; SIR2 L7; SIR2 like protein 7; Sir2 related protein type 7; SIR2, S.CEREVISIAE, HOMOLOG-LIKE 7; SIR2-like protein 7; SIR2L 7; SIR2L7; SIRT 7; SIR7_HUMAN; Sirtuin 7; Sirtuin type 7; Sirtuin7. |
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Specific References (3) | bs-5973R has been referenced in 3 publications.
[IF=3.06] Wronska, A., et al. "Age-Related Changes in Sirtuin 7 Expression in Calorie-Restricted and Refed Rats." Gerontology (2015). WB ;
[IF=2.65] Deng Disi. et al. Protective Effect of XinJiaCongRongTuSiZiWan on the Reproductive Toxicity of Female Rats Induced by Triptolide. EVID-BASED COMPL ALT. 2022;2022:3642349 IHC ; Rat.
[IF=1.11] Takumida, Masaya, Hiroshi Takumida, and Matti Anniko. "Localization of sirtuins in the mouse inner ear." Acta Oto-Laryngologica 0 (2014): 1-8. IHC-F ; Mouse.
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| 研究領(lǐng)域 | 腫瘤 心血管 免疫學 染色質(zhì)和核信號 信號轉(zhuǎn)導(dǎo) 細胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶 結(jié)合蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Pig,Chicken,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 45kDa |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SIRT7: 231-330/400 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
SIRT7 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. Function: NAD-dependent protein deacetylase. Required to restore the transcription of ribosomal RNA (rRNA) at the exit from mitosis. Promotes the association of RNA polymerase I with the rDNA promoter region and coding region. Stimulates transcription activity of the RNA polymerase I complex. Subunit: Interacts with UBTF and the RNA polymerase I complex. Interacts with histone H2A and/or histone H2B. Subcellular Location: Cytoplasm. Nucleus, nucleolus. Note=Located close to the nuclear membrane when in the cytoplasm. Associated with chromatin. Associated with rDNA promoter and transcribed region. Associated with nucleolar organizer regions during mitosis. Post-translational modifications: Phosphorylated during mitosis (Probable). Similarity: Belongs to the sirtuin family. Class IV subfamily. Contains 1 deacetylase sirtuin-type domain. SWISS: Q9NRC8 Gene ID: 51547 Database links: Entrez Gene: 51547 Human Entrez Gene: 209011 Mouse Omim: 606212 Human SwissProt: Q9NRC8 Human SwissProt: Q8BKJ9 Mouse Unigene: 514636 Human Unigene: 292957 Mouse |
| 產(chǎn)品圖片 |
Sample:Small intestine (Mouse)Lysate at 40 ug
Primary: Anti-SIRT7(bs-5973R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 45kD
Observed band size: 50kD
Sample:Lymph node (Mouse)Lysate at 40 ug
Primary: Anti-SIRT7(bs-5973R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 45kD
Observed band size: 50kD
Tissue/cell: mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SIRT7 Polyclonal Antibody, Unconjugated(bs-5973R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: mouse splenocytes(blue)
Isotype Control Antibody: Rabbit IgG(orange) ;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue),
Dilution: 1:100 in 1 X PBS containing 0.5% BSA ;
Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
