產(chǎn)品編號 | bs-5939R |
英文名稱 | Rabbit Anti-PBOV1 antibody |
中文名稱 | 前列腺癌和乳腺癌高表達(dá)蛋白抗體 |
別 名 | PBOV 1; PBOV-1; Prostate and breast cancer overexpressed gene 1 protein; UROC28; Prostate and breast cancer overexpressed 1; UC28.PBOV1_HUMAN |
研究領(lǐng)域 | 腫瘤 免疫學(xué) 染色質(zhì)和核信號 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | Human |
產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 15kDa |
細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human PBOV1: 31-130/135 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
This gene is overexpressed in prostate and breast cancer. Subcellular Location: Cytoplasm. Nucleus. Tissue Specificity: Expressed in colon, prostate, small intestine, testis and spleen, with lower expression in thymus, ovary, and peripheral blood leukocytes. Up-regulated expression in prostate, breast, and bladder cancer, but not in lung and colon cancer. SWISS: Q9GZY1 Gene ID: 59351 Database links: Entrez Gene: 59351 Human Omim: 605669 Human SwissProt: Q9GZY1 Human Unigene: 302016 Human PBOV1蛋白,主要表達(dá)在前列腺癌和乳腺癌等。 |
產(chǎn)品圖片 |
Tissue/cell: human cervical cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PBOV1 Polyclonal Antibody, Unconjugated(bs-5939R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PBOV1) Polyclonal Antibody, Unconjugated (bs-5939R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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