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Rabbit Anti-MMP-2  antibody (bs-4599R)  
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產(chǎn)品編號(hào) bs-4599R
英文名稱 Rabbit Anti-MMP-2  antibody
中文名稱 基質(zhì)金屬蛋白酶2抗體
別    名 MMP2; MMP 2; 72 kDa gelatinase; 72kD type IV collagenase; CLG 4; CLG 4A; CLG4; CLG4A; Collagenase Type 4 alpha; Collagenase type IV A; Gelatinase A; Gelatinase alpha; Gelatinase neutrophil; Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase; Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); Matrix Metalloproteinase 2; Matrix metalloproteinase II; MMP 2; MMP II; MONA; Neutrophil gelatinase; TBE 1.  
Specific References  (23)     |     bs-4599R has been referenced in 23 publications.
[IF=9.261] Wei, Tao. et al. Two distinct males absent on the first (MOF)-containing histone acetyltransferases are involved in the epithelial–mesenchymal transition in different ways in human cells. CELL MOL LIFE SCI. 2022 May;79(5):1-18  WB ;  Human.  
[IF=7.43] Wang, Jing, et al. "Phosphorylation-dependent regulation of ALDH1A1 by Aurora kinase A: insights on their synergistic relationship in pancreatic cancer." BMC biology 15.1 (2017): 10.  WB ;  Human.  
[IF=6.491] Nikhil K et al. Identification of LIMK2 as a Therapeutic Target in Castration Resistant Prostate Cancer. Cancer Lett. 2019 Apr 28;448:182-196.  WB&IHC ;  Mouse.  
[IF=5.83] Fan, Gaochao, et al. "Ultrasensitive photoelectrochemical immunoassay for matrix metalloproteinase-2 detection based on CdS: Mn/CdTe co-sensitized TiO2 nanotubes and signal amplification of SiO2@ Ab2 conjugates." Analytical Chemistry (2014).  other ;  
[IF=5.572] Liu H et al. Anti-tubulin agent vinorelbine inhibits metastasis of cancer cells by regulating epithelial-mesenchymal transition. Eur J Med Chem. 2020 Aug 15;200:112332.  WB ;  Human.  
[IF=5.295] Yue-hua Liu. et al. ANKRD49 promotes the invasion and metastasis of lung adenocarcinoma via a P38/ATF-2 signalling pathway. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2022 Jun 30  IHC,WB ;  Human.  
[IF=5.125] Ashley A. Thurstin. et al. Tissue Inhibitor of Metalloproteinase 4 (Timp4) Deletion in Mice Impacts Maternal Cardiac Function during Pregnancy and Postpartum. AM J PHYSIOL-HEART C. 2022 Dec;(Articles in Press):  WB ;  Mouse.  
[IF=4.43] Gasparski et al. Transient mechanical strain promotes the maturation of invadopodia and enhances cancer cell invasionin vitro. (2017) J.Cell.Sci. 130:1965-1978  WB ;  Human.  
[IF=4.43] Wang et al. The Aurora-A-Twist1 axis promotes highly aggressive phenotypes in pancreatic carcinoma. (2017) J.Cell.Sci. 130:1078-1093  WB ;  Human.  
[IF=4.26] Bi et al. Peripheral serotonin regulates postoperative intra-abdominal adhesion formation in mice. (2017) Sci.Rep. 7:10001  IHC ;  Mouse.  
[IF=4.175] Jia Guo. et al. Circ_0000140 restrains the proliferation, metastasis and glycolysis metabolism of oral squamous cell carcinoma through upregulating CDC73 via sponging miR-182-5p. Cancer Cell Int. 2020 Dec;20(1):1-12  WB ;  Human.  
[IF=3.457] Gao H et al.Salvanic acid B inhibits myocardial fibrosis through regulating TGF-β1/Smad signaling pathway.(2018) Biomed. Pharmacother 110  WB ;  Mouse.  
[IF=3.195] Wang Wenjun. et al. LncRNA NEAT1 antagonizes the inhibition of melanoma proliferation, migration, invasion and EMT by Polyphyllin B. N-S ARCH PHARMACOL. 2023 Apr;:1-12  IHC ;  Mouse.  
[IF=3.072] Xue Y et al. miR‐205‐5p inhibits psoriasis‐associated proliferation and angiogenesis: Wnt/β‐catenin and mitogen‐activated protein kinase signaling pathway are involved. J Dermatol . 2020 Aug;47(8):882-892.  WB ;  Mouse&Human.  
[IF=3.011] Boyu Chen. et al. Study of the effects of rabbit scleral fibroblasts on cellular biomechanical properties and MMP-2 expression using two modes of riboflavin/ultraviolet A wave collagen cross-linking. Exp Eye Res. 2021 Jul;:108695  WB ;  Rabbit.  
[IF=2.625] Shen S et al. The role of CD44 in Tumor‐initiating Cells of Salivary Gland Pleomorphic Adenoma: more than a surface biomarker. Oral Dis. 2020 Jan 11.  WB ;  Mouse.  
[IF=2.447] Xing Chen. et al. Transplantation of decellularized and lyophilized amniotic membrane inhibits endometrial fibrosis by regulating connective tissue growth factor and tissue inhibitor of matrix metalloproteinase?2. Exp Ther Med. 2021 Sep;22(3):1-8  IHC ;  Rat.  
[IF=2.25] Yang X et al.Silencing of Astrocyte elevated gene-1 (AEG-1) inhibits proliferation, migration and invasion, and promotes apoptosis in pancreatic cancer cells. (2018)Biochem. Cell Biol.  WB ;  Human.  
[IF=1.871] Liu B et al. Interleukin-8 promotes prostate cancer bone metastasis through upregulation of bone sialoprotein. Oncol Lett. 2019 May;17(5):4607-4613.  WB ;  Human.  
[IF=1.837] Yang Zhang. et al. Plumbagin Inhibits Proliferation, Migration, and Invasion of Retinal Pigment Epithelial Cells Induced by FGF-2. Tissue Cell. 2021 Oct;72:101547  WB ;  Human.  
[IF=1.664] Li W et al. Association of the invasiveness of colon cancer with the expression of C/EBPα. Oncol Lett. 2019 Feb;17(2):1974-1979.  WB ;  Human.  
[IF=1.06] Cavdar, Z., et al. "The effects of alpha-lipoic acid on MMP-2 and MMP-9 activities in a rat renal ischemia and re-perfusion model." Biotechnic & Histochemistry 0 (2013): 1-11.  IHC-P ;  Rat.  
[IF=1.03] Liu, Jianfeng, et al. "RECK is involved in intimal hyperplasia in carotid arteries: A new insight in the prevention of restenosis after vascular angioplasty."Annals of Vascular Surgery (2015).  WB ;  Human.  
研究領(lǐng)域 腫瘤  免疫學(xué)  神經(jīng)生物學(xué)  合成與降解  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse,Rat,Rabbit (predicted: Pig,Sheep,Cow,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg/Test,ICC/IF=1:100,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 62kDa
細(xì)胞定位 細(xì)胞核 細(xì)胞漿 細(xì)胞膜 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human MMP-2: 401-500/662 
亞    型
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]

Function:
Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14.
PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
Isoform 2: Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.

Subunit:
Interacts (via the C-terminal hemopexin-like domains-containing region) with the integrin alpha-V/beta-3; the interaction promotes vascular invasion in angiogenic vessels and melamoma cells. Interacts (via the C-terminal PEX domain) with TIMP2 (via the C-terminal); the interaction inhibits the degradation activity. Interacts with GSK3B.

Subcellular Location:
Isoform 1: Secreted, extracellular space, extracellular matrix. Membrane. Nucleus. Note=Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
Isoform 2: Cytoplasm. Mitochondrion.

Tissue Specificity:
Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.

Post-translational modifications:
Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.

DISEASE:
Multicentric osteolysis, nodulosis, and arthropathy (MONA) [MIM:259600]: An autosomal recessive syndrome characterized by severe multicentric osteolysis with predominant involvement of the hands and feet. Additional features include coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. {ECO:0000269|PubMed:11431697, disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Belongs to the peptidase M10A family. {ECO:0000305}.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin repeats.

SWISS:
P08253

Gene ID:
4313

Database links:

Entrez Gene: 4313 Human

Entrez Gene: 17390 Mouse

Entrez Gene: 81686 Rat

Omim: 120360 Human

SwissProt: P08253 Human

SwissProt: P33434 Mouse

SwissProt: P33436 Rat

Unigene: 513617 Human

Unigene: 29564 Mouse

Unigene: 6422 Rat



基質(zhì)金屬蛋白酶(matrix metalloproteinases, MMPs)MMP2是一族依賴鋅離子而降解各種細(xì)胞外基質(zhì)的蛋白酶,亦稱IV型膠原酶或稱明膠酶A,其主要功能為降解IV型膠原,因而它在腫瘤細(xì)胞突破基底膜屏障和浸潤轉(zhuǎn)移中起重要作用。目前主要用于各種惡性腫瘤(如乳腺癌、胃腸道癌、卵巢癌、膀胱癌等)中的基底膜檢測與轉(zhuǎn)移浸潤的研究。
產(chǎn)品圖片
Sample: A431(Human) Cell Lysate at 30 ug Mcf-7(Human) Cell Lysate at 30 ug Primary: Anti-MMP-2 (bs-4599R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 62 kD
Sample: SY5Y (Human) Cell Lysate at 30 ug U2os (Human) Cell Lysate at 30 ug U251 (Human) Cell Lysate at 30 ug Siha (Human) Cell Lysate at 30 ug Primary: Anti- MMP-2 (bs-4599R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 60 kD
Paraformaldehyde-fixed, paraffin embedded (mouse placenta tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MMP-2) Polyclonal Antibody, Unconjugated (bs-4599R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MMP-2) Polyclonal Antibody, Unconjugated (bs-4599R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: U-87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (MMP2)polyclonal Antibody, Unconjugated (bs-4599R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control (blue line): Hela (fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti- MMP2 antibody (bs-4599R),Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.
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