| 產(chǎn)品編號 | bs-4275R |
| 英文名稱 | Rabbit Anti-EBF3 antibody |
| 中文名稱 | 微管相關蛋白EB家族3抗體 |
| 別 名 | APC binding protein; EB 3; EB1 protein family member 3; EB3; EBF 3; EBF3; EBF3 S; End binding protein 3; End-binding protein 3; MAPRE 3; MAPRE3; MARE3_HUMAN; Member 3; Microtubule Associated Protein; Microtubule associated protein RP/EB family member 3; Microtubule-associated protein RP/EB family member 3; OTTHUMP00000200910; RP 3; RP/EB Family; RP3. |
| 研究領域 | 腫瘤 細胞生物 免疫學 信號轉導 細胞周期蛋白 細胞骨架 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rat (predicted: Human,Mouse,Rabbit,Pig,Cow,Chicken,Dog) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 25kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EB3: 75-185/281 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration. Function: Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration (By similarity). Subunit: Interacts with APC2 (By similarity). Interacts with DCTN1 and SRCIN1. Binds to the C-terminal domain of APC. Binds monomeric and polymerized tubulin. Subcellular Location: Cytoplasm, cytoskeleton. Tissue Specificity: Predominantly expressed in brain and muscle. Similarity: Belongs to the MAPRE family. Contains 1 CH (calponin-homology) domain. Contains 1 EB1 C-terminal domain. SWISS: Q9H4W6 Gene ID: 253738 Database links: Entrez Gene: 253738 Human Entrez Gene: 13593 Mouse Omim: 607407 Human SwissProt: Q9H4W6 Human SwissProt: O08791 Mouse Unigene: 591374 Human Unigene: 258708 Mouse |
| 產(chǎn)品圖片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-EB3 Polyclonal Antibody, Unconjugated(bs-4275R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
