| 產(chǎn)品編號(hào) | bs-5922R |
| 英文名稱(chēng) | Rabbit Anti-PGRMC antibody |
| 中文名稱(chēng) | 孕激素受體膜相關(guān)元件抗體 |
| 別 名 | HPR6.6; Membrane associated progesterone receptor component 1; y mPR; PGRMC1; PGRMC 1; PGRMC-1; Progesterone receptor membrane component 1. |
| 研究領(lǐng)域 | 腫瘤 心血管 免疫學(xué) 生長(zhǎng)因子和激素 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Rabbit,Pig,Cow,Chicken) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 21kDa |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PGRMC: 75-170/195 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
PGRMC1 is a progesterone receptor component of 195 aa with a 26 residue N terminal hydrophobic region and no N terminal glycosylation site. It has an overall homology of 50% with PGRMC2 but their C terminal regions have a 68% homology, including a highly conserved 58 residue sequence.PGRMC1 is a heme bind proteins that is up-regulated in several cancers. In breast tumors, PGRMC1 is associated with hypoxia estrogen receptor-negative status. PGRMC1 is also shown to stimulate AKT phosphorylation and is known to stabilise the EGFR receptor tyrosine kinase. Function: Receptor for progesterone (By similarity). Subcellular Location: Microsome membrane; Single-pass membrane protein (By similarity). Endoplasmic reticulum membrane; Single-pass membrane protein (By similarity). Tissue Specificity: Widely expressed, with highest expression in liver and kidney. Similarity: Belongs to the cytochrome b5 family. MAPR subfamily. Contains 1 cytochrome b5 heme-binding domain. SWISS: O00264 Gene ID: 10857 Database links: Entrez Gene: 10857 Human Entrez Gene: 53328 Mouse Omim: 300435 Human SwissProt: O00264 Human SwissProt: O55022 Mouse Unigene: 90061 Human Unigene: 9052 Mouse Unigene: 102356 Rat |
| 產(chǎn)品圖片 |
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PGRMC Polyclonal Antibody, Unconjugated(bs-5922R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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