| 產品編號 | bs-5600R |
| 英文名稱 | Rabbit Anti-phospho-TSC1 (Ser505) antibody |
| 中文名稱 | 磷酸化結節(jié)性硬化癥蛋白1抗體 |
| 別 名 | TSC1(phospho S505); TSC1(phospho Ser505); LAM; Hamartin; Tuberous sclerosis 1 protein; TSC1_HUMAN; KIAA0243; TSC; Tuberous sclerosis 1. |
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Specific References (1) | bs-5600R has been referenced in 1 publications.
[IF=4.8] Cao, Jiaxue, et al. "DNA methylation Landscape of body size variation in sheep." Scientific reports 5 (2015). WB ; Sheep.
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| 產品類型 | 磷酸化抗體 |
| 研究領域 | 腫瘤 細胞生物 染色質和核信號 細胞周期蛋白 轉錄調節(jié)因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 128kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human TSC1 around the phosphorylation site of Ser505: FD(p-S)PF |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Hamartin, or TSC1, is a suspected tumor suppressor implicated in the disease tuberous sclerosis 1. It is a negative regulator of cell division controlling the transition from G0/G1 to S phase, and it seems to act through the phosphatidylinositol 3 kinase/Akt pathway. TSC1 interacts with tuberin m(TSC2), which is thought to be a GAP (GTPase Activating Protein) for the Rap1 and Rab5 small G Proteins. The Hamartin/Tuberin complex has been shown to inhibit mTor. Hamartin has also been shown to interact with ERM (Ezrin-Radixin-Moesin) proteins and with F-actin, suggesting a role for TSC proteins in modulation of cell adhesion and morphology. Function: In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Seems not to be required for TSC2 GAP activity towards RHEB. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. Subunit: Interacts with TSC2, leading to stabilize TSC2. In the absence of TSC2, TSC1 self-aggregates. Interacts with DOCK7. Interacts with FBXW5 and TBC1D7. Subcellular Location: Cytoplasm. Membrane; Peripheral membrane protein. Note=At steady state found in association with membranes. Tissue Specificity: Highly expressed in skeletal muscle, followed by heart, brain, placenta, pancreas, lung, liver and kidney. Also expressed in embryonic kidney cells. Post-translational modifications: Phosphorylation at Ser-505 does not affect interaction with TSC2. Phosphorylated upon DNA damage, probably by ATM or ATR. DISEASE: Defects in TSC1 are the cause of tuberous sclerosis type 1 (TSC1) [MIM:191100]. It is an autosomal dominant multi-system disorder that affects especially the brain, kidneys, heart, and skin. TS1C is characterized by hamartomas (benign overgrowths predominantly of a cell or tissue type that occurs normally in the organ) and hamartias (developmental abnormalities of tissue combination). Clinical symptoms can range from benign hypopigmented macules of the skin to profound mental retardation with intractable seizures to premature death from a variety of disease-associated causes. Defects in TSC1 may be a cause of focal cortical dysplasia of Taylor balloon cell type (FCDBC) [MIM:607341]. FCDBC is a subtype of cortical displasias linked to chronic intractable epilepsy. Cortical dysplasias display a broad spectrum of structural changes, which appear to result from changes in proliferation, migration, differentiation, and apoptosis of neuronal precursors and neurons during cortical development. SWISS: Q92574 Gene ID: 7248 Database links: Entrez Gene: 7248 Human Entrez Gene: 64930 Mouse Omim: 605284 Human SwissProt: Q92574 Human SwissProt: Q9EP53 Mouse Unigene: 370854 Human Unigene: 224354 Mouse Unigene: 205837 Rat ????結節(jié)性硬化癥為常染色體顯性遺傳，也常見散發(fā)病例。是腫瘤抑制基因，基因產物分別為Hamartin和tuberin，兩者均調節(jié)細胞生長。 ????結節(jié)性硬化癥(tuberous sclerosis)又稱結節(jié)性腦硬化，Bourneville病。本病可歸類于神經皮膚綜合征(亦稱斑痣性錯構瘤病)，是源于外胚層的器官發(fā)育異常所致，病變累及神經系統、皮膚和眼，也可累及中胚層，內胚層器官如心、肺、骨，腎和胃腸等。皮脂腺瘤是皮膚神經末梢、增生的結締組織和血管組成，視網膜可見膠質瘤、神經節(jié)細胞瘤，心、腎、肺、肝臟等也可發(fā)生腫瘤。 ????而神經膠質增生性硬化結節(jié)廣泛發(fā)生于大腦皮質、白質、基底節(jié)和室管膜下，常伴鈣質沉積，可出現一位癥及血管增生等，出現癲癇發(fā)作及智能減退為特征。 |
| 產品圖片 |
Sample:
NIH/3T3(Mouse) Cell Lysate at 30 ug
Primary: Anti-phospho-TSC1(Ser505) (bs-5600R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 128 kD
Observed band size: 125 kD
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TSC1(Ser505)) Polyclonal Antibody, Unconjugated (bs-5600R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
