| 產(chǎn)品編號(hào) | bs-5472R |
| 英文名稱 | Rabbit Anti-phospho-MCAM (Tyr641) antibody |
| 中文名稱 | 磷酸化黑色素瘤細(xì)胞粘附分子CD146抗體 |
| 別 名 | MCAM (phospho Y641); A32 antigen; CD 146; CD146; CD146 antigen; Cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; MCAM; Melanoma adhesion molecule; Melanoma associated antigen A32; Melanoma associated antigen MUC18; Melanoma associated glycoprotein MUC18; Melanoma cell adhesion molecule; MelCAM; MUC 18; MUC18; S endo 1; S endo 1 endothelial associated antigen; Sendo 1 endothelial associated antigen; Sendo1; MUC18_HUMAN. |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 71kDa |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human MCAM around the phosphorylation site of Tyr641: EK(p-Y)ID <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
MCAM (MUC18 antigen, CD146), a member of the immuoglobulin superfamily, is an intrinsic membrane glycoprotein of 110-120 kDa found on the surface of endothelial cells, bone marrow fibroblasts and various melanomas. MCAM (Melanoma adhesion molecule) has been used as a marker of tumor progression in human melanoma because expression in those tumors correlates strongly with poor prognosis and the development of metastic disease. In addition, a number of human T, B and myeloid leukemic cell lines seem to express MCAM. The close structural relationship with N-CAM and related molecules suggests that MCAM may be also a developmentally regulated cell adhesion. Function: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration. Subcellular Location: Membrane. Tissue Specificity: Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis. Similarity: Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 2 Ig-like V-type (immunoglobulin-like) domains. SWISS: P43121 Gene ID: 4162 Database links: Entrez Gene: 4162 Human Entrez Gene: 84004 Mouse Omim: 155735 Human SwissProt: P43121 Human SwissProt: Q8R2Y2 Mouse Unigene: 599039 Human Unigene: 275003 Mouse Unigene: 2694 Rat CD146分子主要是選擇性的在腫瘤血管內(nèi)皮細(xì)胞中表達(dá)較高。 |
| 產(chǎn)品圖片 |
Sample:
Siha(Human) Cell Lysate at 30 ug
Primary: Anti- phospho-MCAM (Tyr641) (bs-5472R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 71 kD
Observed band size: 118 kD
Sample:
Lane 1: Mouse Placenta tissue lysates
Lane 2: Mouse Kidney tissue lysates
Lane 3: Human Huvec cell lysates
Lane 4: Human Hela cell lysates
Lane 5: Human U2OS cell lysates
Lane 6: Human U87MG cell lysates
Primary: Anti-phospho-MCAM (Tyr641) (bs-5472R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 71 kDa
Observed band size: 117 kDa
|
