| 產(chǎn)品編號 | bs-0225P |
| 英文名稱 | KLH |
| 中文名稱 | 血藍蛋白/鑰孔血藍蛋白（進口） |
| 別 名 | Keyhole limpet; keyhole limpet hemocyanin; Hemocyanin from Megathura crenulata (keyhole limpet); Hemocyanin from Limulus polyphemus hemolymph; hemocyanin; haemocyanin. 載體蛋白; 血蘭蛋白; 鑰孔戚血藍蛋白; 血藍蛋白(KLH); 血清朊; 血藍素; 匙孔嘁血藍蛋白; |
| 性 狀 | Lyophilized |
| 物 種 | Keyhole limpet |
| 序 列 | Purified native protein |
| 內(nèi)毒素 | Not analyzed |
| 活性 | Not tested |
| 保存條件 | Stored at 2-8℃. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品介紹 |
Keyhole limpet hemocyanin (KLH) is used extensively as a carrier protein in the production of antibodies for research, biotechnology and therapeutic applications. Haptens are substances with a low molecular weight such as peptides, small proteins and drug molecules that are generally not immunogenic and require the aid of a carrier protein to stimulate a response from the immune system in the form of antibody production. KLH is the most widely employed carrier protein for this purpose. KLH is an effective carrier protein for several reasons. Its large size and numerous epitopes generate a substantial immune response, and abundance of lysine residues for coupling haptens allows a high hapten:carrier protein ratio, increasing the likelihood of generating hapten-specific antibodies. In addition, because KLH is derived from the limpet, a gastropod, it is phylogenetically distant from mammalian proteins, thus reducing false positives in immunologically-based research techniques in mammalian model organisms. KLH can also be a challenging molecule to work with because of its propensity to aggregate and precipitate. Aggregates remain immunogenic, but limit the ability to conjugate haptens, and are difficult to manipulate in the laboratory. A high-quality KLH preparation with clear opalescent blue color is the best indicator of KLH solubility. SWISS: Q10583 Gene ID: 110853033 CAS：9013-72-3 分子量：8000-9000kDa Type: Type VIII Form: lyophilized powder Composition: Protein, ≥85% biuret Copper content: 0.10-0.20% (ICP-atomic emission) 來源：Limulus polyphemus hemolymph 外觀：白色帶微藍色凍干粉 介紹：血藍蛋白是在某些軟體動物、節(jié)肢動物(蜘蛛和甲殼蟲)的血淋巴中發(fā)現(xiàn)的一種游離的藍 色呼吸色素。血藍蛋白含兩個直接連接多肽鏈的銅離子，與含鐵的血紅蛋白類似，它易于氧結(jié)合，也易與氧解離，是已知的惟一可與氧可逆結(jié)合的銅蛋白，氧化時呈青綠色，還原時呈白色。節(jié)肢動 物中血藍蛋白的含量比軟體動物的含量要高。具有載氧功能、抗病毒活性、抗菌活性、凝血功能。 用途：血藍蛋白是具有高度免疫原性的蛋白大分子，作為載體蛋白用于免疫原的制備，交聯(lián)于半抗原和其他抗原，增強它們的免疫原性。 SIGMA：H1757 |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
