| 產(chǎn)品編號 | bs-5097R |
| 英文名稱 | Rabbit Anti-NNT antibody |
| 中文名稱 | 煙酰胺核苷酸轉(zhuǎn)氫酶抗體 |
| 別 名 | NAD(P) transhydrogenase mitochondrial; nicotinamide nucleotide transhydrogenase; NNTM; Pyridine nucleotide transhydrogenase; NNTM_HUMAN. |
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Specific References (2) | bs-5097R has been referenced in 2 publications.
[IF=4.331] Salerno AG et al. Lack of mitochondrial NADP (H)-transhydrogenase expression in macrophages exacerbates atherosclerosis in hypercholesterolemic mice. Biochem J. 2019 Dec 23;476(24):3769-3789. WB ; Mouse.
[IF=0] Roider, Elisabeth Maria, and David Erich Fisher. "METHODS AND COMPOSITIONS FOR ENHANCING SKIN PIGMENTATION." U.S. Patent No. 20,160,136,070. 19 May 2016. other ; Human.
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| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 線粒體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Dog (predicted: Mouse,Rat,Rabbit,Pig,Cow,Chicken,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 109kDa |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NNT: 981-1086/1086 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Nicotinamide nucleotide transhydrogenase (NNT) is an integral protein of the inner mitochondrial membrane.It couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane.
Function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane. May play a role in reactive oxygen species (ROS) detoxification in the adrenal gland. Subunit: Homodimer Subcellular Location: Mitochondrion inner membrane; Multi-pass membrane protein; Matrix side Tissue Specificity: Widely expressed with expression most readily detectable in adrenal, heart, kidney, thyroid and adipose tissues. DISEASE: Defects in NNT are the cause of glucocorticoid deficiency type 4 (GCCD4) [MIM:614736]. A rare, potentially lethal, autosomal recessive disorder characterized by resistance to ACTH action on the adrenal cortex, adrenal insufficiency and an inability of the adrenal cortex to produce cortisol. It usually presents in the neonatal period or in early childhood with episodes of hypoglycemia and other symptoms related to cortisol deficiency, including failure to thrive, recurrent illnesses or infections, convulsions, and shock. In a small number of patients hypoglycemia can be sufficiently severe and persistent that it leads to serious long-term neurological damage or death. The diagnosis is readily confirmed with a low plasma cortisol measurement in the presence of an elevated ACTH level, and normal aldosterone and plasma renin measurements. Similarity: In the N-terminal section; belongs to the AlaDH/PNT family. In the C-terminal section; belongs to the PNT beta subunit family. SWISS: Q13423 Gene ID: 23530 Database links: Entrez Gene: 23530 Human SwissProt: Q13423 Human SwissProt: Q61941 Mouse Unigene: 5120 Cow Unigene: 482043 Human Unigene: 195803 Mouse |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NNT) Polyclonal Antibody, Unconjugated (bs-5097R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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