| 產品編號 | bs-5041R |
| 英文名稱 | Rabbit Anti-BDH1 antibody |
| 中文名稱 | 3-羥丁酸脫氫酶抗體 |
| 別 名 | 3 hydroxybutyrate dehydrogenase (heart, mitochondrial); BDH; BDH-1; D beta hydroxybutyrate dehydrogenase, mitochondrial; 3-hydroxybutyrate dehydrogenase; D-beta-hydroxybutyrate dehydrogenase; mitochondrial; BDH_HUMAN . |
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Specific References (2) | bs-5041R has been referenced in 2 publications.
[IF=4.225]
[IF=4.225] Li Huihui. et al. Trimetazidine Ameliorates Myocardial Metabolic Remodeling in Isoproterenol-Induced Rats Through Regulating Ketone Body Metabolism via Activating AMPK and PPAR α. Front Pharmacol. 2020 Aug;11:1255 WB ; Rat.
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| 研究領域 | 腫瘤 細胞生物 免疫學 轉錄調節(jié)因子 轉運蛋白 線粒體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Rabbit,Horse) |
| 產品應用 | WB=1:500-2000,Flow-Cyt=3ug/Test,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 33kDa |
| 細胞定位 | 細胞漿 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BDH1: 241-343/343 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a member of the short-chain dehydrogenase/reductase gene family. The encoded protein forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific requirement for phosphatidylcholine for optimal enzymatic activity. The encoded protein catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the two major ketone bodies produced during fatty acid catabolism. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]. Subunit: Homotetramer. Subcellular Location: Mitochondrion matrix. Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. SWISS: Q02338 Gene ID: 622 Database links: Entrez Gene: 622 Human Entrez Gene: 71911 Mouse Omim: 603063 Human SwissProt: Q02338 Human SwissProt: Q80XN0 Mouse Unigene: 274539 Human Unigene: 293470 Mouse |
| 產品圖片 |
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-BDH1 antibody (bs-5041R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min atroom temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
