| 產品編號 | bs-4029R |
| 英文名稱 | Rabbit Anti-CA9 antibody |
| 中文名稱 | 碳酸酐酶9抗體 |
| 別 名 | CA IX; CA-IX; Carbonic anhydrase 9; CAH9_HUMAN; CAIX; Carbonate dehydratase IX; Carbonic anhydrase IX; Carbonic dehydratase; G250; Membrane antigen MN; MN; P54/58N; P54/58N; pMW1; RCC associated protein G250; RCC-associated antigen G250; Renal cell carcinoma associated antigen G250; Renal cell carcinoma-associated antigen G250. |
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Specific References (3) | bs-4029R has been referenced in 3 publications.
[IF=10.383] Jiabao Ling. et al. Designing Lactate Dehydrogenase-Mimicking SnSe Nanosheets To Reprogram Tumor-Associated Macrophages for Potentiation of Photothermal Immunotherapy. ACS APPL MATER INTER. 2022;14(24):27651–27665 WB ; Mouse.
[IF=4.493] Qin Wentao. et al. Systematic Construction and Validation of a Novel Ferroptosis-Related Gene Model for Predicting Prognosis in Cervical Cancer. J IMMUNOL RES. 2022;2022:2148215 IHC ; Human.
[IF=2.88] Gogiraju, Rajinikanth, et al. "Endothelial p53 Deletion Improves Angiogenesis and Prevents Cardiac Fibrosis and Heart Failure Induced by Pressure Overload in Mice." Journal of the American Heart Association 4.2 (2015): e001770. IHC-F ; Mouse.
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| 研究領域 | 腫瘤 細胞生物 免疫學 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Pig,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:25,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 46kDa |
| 細胞定位 | 細胞核 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Carbonate dehydratase IX: 251-350/459 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jul 2008] Function: Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. Subunit: Forms oligomers linked by disulfide bonds. Subcellular Location: Nucleus. Nucleus, nucleolus. Cell membrane; Single-pass type I membrane protein. Cell projection, microvillus membrane; Single-pass type I membrane protein. Note=Found on the surface microvilli and in the nucleus, particularly in nucleolus. Tissue Specificity: Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. Similarity: Belongs to the alpha-carbonic anhydrase family. SWISS: Q16790 Gene ID: 768 Database links: Entrez Gene: 768 Human Entrez Gene: 230099 Mouse Omim: 603179 Human SwissProt: Q16790 Human SwissProt: Q8VHB5 Mouse Unigene: 63287 Human Unigene: 283682 Mouse ????碳酸酐酶9（carbonic anhydrase IX，CA IX）是新發(fā)現(xiàn)的碳酸酐酶家族異構體之一，是由酸性氨基酸組成的跨膜糖蛋白，在調控細胞增殖、轉化方面有重要作用。 ????碳酸酐酶9能催化CO2水解為碳酸和水，參與機體的酸堿平衡，調節(jié)細胞內外pH值，易導致腫瘤的生長和轉移，目前也是子宮腫瘤新的標記物。 |
| 產品圖片 |
Sample:
Stomach (Rat) Lysate at 40 ug
Primary: Anti-Carbonate dehydratase IX (bs-4029R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 48/58 kD
Sample:
Stomach (Mouse) Lysate at 40 ug
Primary: Anti-Carbonate dehydratase IX (bs-4029R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 48 kD
Paraformaldehyde-fixed, paraffin embedded (human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CA9 ) Polyclonal Antibody, Unconjugated (bs-4029R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CA9 ) Polyclonal Antibody, Unconjugated (bs-4029R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (CA9) polyclonal Antibody, Unconjugated (bs-4029R) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
