| 產(chǎn)品編號 | bs-3957R |
| 英文名稱 | Rabbit Anti-NDUFA10 antibody |
| 中文名稱 | NADH氧化還原酶輔酶10抗體 |
| 別 名 | CI 42KD; CI-42k; Complex I-42kD; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 10; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 10 42kDa; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial; NADH ubiquinone oxidoreductase 42 kDa subunit mitochondrial precursor; NADH-ubiquinone oxidoreductase 42 kDa subunit; ndufa10l; MGC5103; NDUAA_HUMAN. |
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Specific References (1) | bs-3957R has been referenced in 1 publications.
[IF=7.129] Wang Yang. et al. Benzo[a]pyrene inhibits testosterone biosynthesis via NDUFA10-mediated mitochondrial compromise in mouse Leydig cells: Integrating experimental and in silico toxicological approaches. ECOTOX ENVIRON SAFE. 2022 Oct;244:114075 WB ; Mouse.
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| 研究領域 | 腫瘤 細胞生物 免疫學 轉錄調(diào)節(jié)因子 結合蛋白 線粒體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 36kDa |
| 細胞定位 | 細胞漿 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NDUFA10: 201-300/355 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
NDUFA10 protein belongs to the complex I 42kDa subunit family. Mammalian complex I is the first enzyme complex in the electron transport chain of mitochondria. It is composed of 45 different subunits. This protein is a component of the hydrophobic protein fraction and has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. Function: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Subunit: Complex I is composed of 45 different subunits. This a component of the hydrophobic protein fraction. Subcellular Location: Mitochondrion matrix. Similarity: Belongs to the complex I NDUFA10 subunit family. SWISS: O95299 Gene ID: 4705 Database links: UniProtKB/Swiss-Prot: O95299.1 |
| 產(chǎn)品圖片 |
Sample: Heart (Mouse) Lysate at 40 ug
Primary: Anti-NDUFA10 (bs-3957R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 40 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
