產(chǎn)品編號(hào) | bs-3894R |
英文名稱(chēng) | Rabbit Anti-Sulfatase 2 antibody |
中文名稱(chēng) | 硫酸酯酶2蛋白抗體 |
別 名 | Extracellular sulfatase Sulf 2; HSulf2; SULF2; SULF-2; SULF 2. |
研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 染色質(zhì)和核信號(hào) |
抗體來(lái)源 | Rabbit |
克隆類(lèi)型 | Polyclonal |
交叉反應(yīng) | Human,Rat (predicted: Mouse,Rabbit,Pig,Cow,Chicken,Dog) |
產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 93kDa |
細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 細(xì)胞外基質(zhì) |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human Sulfatase 2: 651-750/870 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
Sulfatase 2 exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. It is expressed at highest levels in the ovary, skeletal muscle, stomach, brain, uterus, heart, kidney and placenta. Function: Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. Subcellular Location: Endoplasmic reticulum. Golgi apparatus, Golgi stack. Cell surface. Note=Also localized on the cell surface. Tissue Specificity: Expressed at highest levels in the ovary, skeletal muscle, stomach, brain, uterus, heart, kidney and placenta. Post-translational modifications: The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. SWISS: Q8IWU5 Gene ID: 55959 Database links: Entrez Gene: 55959 Human Entrez Gene: 72043 Mouse SwissProt: Q8IWU5 Human SwissProt: Q8CFG0 Mouse Unigene: 162016 Human Unigene: 1011 Mouse |
產(chǎn)品圖片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Sulfatase 2 Polyclonal Antibody, Unconjugated(bs-3894R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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