| 產(chǎn)品編號(hào) | bs-3713R |
| 英文名稱(chēng) | Rabbit Anti-Phospho-p57 Kip2 (Thr310) antibody |
| 中文名稱(chēng) | 磷酸化周期蛋白依賴(lài)激酶抑制因子1C抗體 |
| 別 名 | p57 Kip2 (phospho T310); p57 Kip2 (phospho Thr310); p57 Kip2 (p-T310); Beckwith Wiedemann syndrome; BWCR; BWS; CDKI; CDKN 1C; CDKN1C; Cyclin dependent kinase inhibitor 1C; Cyclin dependent kinase inhibitor p57; KIP 2; KIP2; p57; p57 Kip 2; p57Kip2; WBS; IMAGE; CDN1C_HUMAN. |
| 產(chǎn)品類(lèi)型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 細(xì)胞周期蛋白 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 35kDa |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human p57 Kip2 around the phosphorylation site of Thr310: EQ(p-T)PR |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene is imprinted, with preferential expression of the maternal allele. The encoded protein is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]. Function: Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non-proliferative state throughout life. Subunit: Interacts with PCNA. Subcellular Location: Nucleus. Tissue Specificity: Expressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. Expressed in the eye. High levels are seen in the placenta while low levels are seen in the liver. DISEASE: Defects in CDKN1C are a cause of Beckwith-Wiedemann syndrome (BWS) [MIM:130650]. BWS is a genetically heterogeneous disorder characterized by anterior abdominal wall defects including exomphalos (omphalocele), pre- and postnatal overgrowth, and macroglossia. Additional less frequent complications include specific developmental defects and a predisposition to embryonal tumors. Defects in CDKN1C are the cause of intrauterine growth retardation, metaphyseal dysplasia, adrenal hypoplasia congenita, and genital anomalies (IMAGE) [MIM:614732]. A rare condition characterized by intrauterine growth restriction, metaphyseal dysplasia, congenital adrenal hypoplasia, and genital anomalies. Patients with this condition may present shortly after birth with severe adrenal insufficiency, which can be life-threatening if not recognized early and commenced on steroid replacement therapy. Other reported features in this condition include, hypercalciuria and/or hypercalcemia, craniosynostosis, cleft palate, and scoliosis. Note=Defects in CDKN1C are involved in tumor formation. Similarity: Belongs to the CDI family. SWISS: P49918 Gene ID: 1028 Database links: Entrez Gene: 1028 Human Entrez Gene: 12577 Mouse Omim: 600856 Human SwissProt: P49918 Human SwissProt: P49919 Mouse Unigene: 106070 Human Unigene: 168789 Mouse Unigene: 162507 Rat p57 Kip-2調(diào)控周期蛋白依賴(lài)蛋白激酶、G1 期, 是細(xì)胞周期蛋白依賴(lài)性激酶(CDK)的抑制蛋白。它通過(guò)調(diào)控細(xì)胞周期進(jìn)程，參與腫瘤細(xì)胞的增殖、分化與凋亡。在多種腫瘤中均發(fā)現(xiàn)p57,kip2表達(dá)異常，在某些腫瘤中是一種獨(dú)立的預(yù)后因素，與腫瘤的發(fā)生、發(fā)展及預(yù)后有著密切關(guān)系。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-p57 Kip2 (Thr310)) Polyclonal Antibody, Unconjugated (bs-3713R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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