| 產(chǎn)品編號 | bs-3428R |
| 英文名稱 | Rabbit Anti-Phospho-Stat2 (Tyr690) antibody |
| 中文名稱 | 磷酸化信號轉(zhuǎn)導(dǎo)和轉(zhuǎn)錄激活因子2抗體 |
| 別 名 | Stat2 (phospho-Tyr690); Stat2 (phospho Y690); Stat2 (phospho Tyr690); p-Stat2 (Tyr690); signal transducers and activators of transduction2; Homo sapiens interferon alpha induced transcriptional activator; interferon alpha induced transcriptional activator; ISGF3; P113; signal transducer and activator of transcription 2 113kD; STAT113; STAT2_HUMAN; p113. |
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Specific References (4) | bs-3428R has been referenced in 4 publications.
[IF=6.448] Collotta D et al. Baricitinib counteracts metaflammation, thus protecting against diet-induced metabolic abnormalities in mice. Mol Metab
.2020 Sep;39:101009. WB ; Mouse.
[IF=6.304] Chen Y et al. Dendritic cells-derived interferon-λ1 ameliorated inflammatory bone destruction through inhibiting osteoclastogenesis. Cell Death Dis. 2020 Jun 2;11(6):414. WB ; Mouse.
[IF=6.291] Changjiang Liu. et al. Cypermethrin triggers YY1-mediated testosterone biosynthesis suppression. Ecotox Environ Safe. 2021 Dec;225:112792 WB ; Rat.
[IF=5.162] Zhuo Han. et al. New insights into Vitamin C function: Vitamin C induces JAK2 activation through its receptor-like transporter SVCT2. Int J Biol Macromol. 2021 Mar;173:379 WB ; Human.
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| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 細(xì)胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 94kDa |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human Stat2 around the phosphorylation site of Tyr690: RK(p-Y)LK |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. Transcription adaptor P300/CBP (EP300/CREBBP) has been shown to interact specifically with this protein, which is thought to be involved in the process of blocking IFN-alpha response by adenovirus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]. Function: Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. Subunit: Interacts with ISGF3G/IRF-9 in the cytoplasm. Heterodimer with STAT1 upon IFN-alpha/beta induced phosphorylation. Interacts with CRSP2 and CRSP6. Interacts with Simian virus 5 protein V and rabies virus phosphoprotein (By similarity). Can form a homodimer upon IFN-alpha induced phosphorylation. Interacts with IFNAR1; the interaction requires the phosphorylation of IFNAR1 at 'Tyr-466'. Interacts with IFNAR2. Interacts with dengue virus NS5; this interaction inhibits the phosphorylation of STAT2, and, when all viral proteins are present (polyprotein), targets STAT2 for degradation. Interacts with human cytomegalovirus/HHV-5 protein UL123; this interaction promotes viral growth. Subcellular Location: Cytoplasm. Nucleus. Note=Translocated into the nucleus upon activation by IFN-alpha/beta. Post-translational modifications: Tyrosine phosphorylated in response to IFN-alpha. Similarity: Belongs to the transcription factor STAT family. Contains 1 SH2 domain. SWISS: P52630 Gene ID: 6773 Database links: Entrez Gene: 6773 Human Entrez Gene: 20847 Mouse Omim: 600556 Human SwissProt: P52630 Human SwissProt: Q9WVL2 Mouse Unigene: 530595 Human Unigene: 293120 Mouse Unigene: 471333 Mouse Unigene: 24237 Rat STAT-信號轉(zhuǎn)導(dǎo)和轉(zhuǎn)錄激活因子蛋白家族是近年來發(fā)現(xiàn)的一類轉(zhuǎn)錄因子.通過JAK-STAT途徑介導(dǎo)許多由細(xì)胞因子誘導(dǎo)的反應(yīng),參與細(xì)胞的信號轉(zhuǎn)導(dǎo)和轉(zhuǎn)錄。 |
| 產(chǎn)品圖片 |
Sample:Brain (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-Stat2 (Tyr690)(bs-3428R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 94kD
Observed band size: 105kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Stat2 (Tyr690)) Polyclonal Antibody, Unconjugated (bs-3428R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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