| 產(chǎn)品編號(hào) | bs-3539R |
| 英文名稱 | Rabbit Anti-Paxillin antibody |
| 中文名稱 | 樁蛋白Paxillin抗體 |
| 別 名 | Paired box protein Pax 1; PAX 1; PAX1; Paxillin alpha; PXN; PXN protein; FLJ16691; FLJ23042; FLJ16691; PAXI_HUMAN; Paxillin; PXN protein. |
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Specific References (2) | bs-3539R has been referenced in 2 publications.
[IF=9.776] Jingwen Dong. et al. “Attractive/adhesion force” dual-regulatory nanogels capable of CXCR4 antagonism and autophagy inhibition for the treatment of metastatic breast cancer. J Control Release. 2022 Jan;341:892 WB,IF,IHC ; Mouse.
[IF=4.268] Jian-xia Wen. et al. Therapeutic Effects and Potential Mechanism of Dehydroevodiamine on N-Methyl-N′-Nitro-N-Nitrosoguanidine-Induced Chronic Atrophic Gastritis. Phytomedicine. 2021 Jun;:153619 WB,IHC ; Rat.
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| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Rabbit,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=3ug/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 68kDa |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Paxillin: 501-591/591 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011] Function: Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Subunit: Binds in vitro to vinculin as well as to the SH3 domain of SRC and, when tyrosine phosphorylated, to the SH2 domain of V-CRK. Isoform beta binds to PTK2/FAK1 but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to PTK2/FAK1. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1. Interacts with PTK2/FAK1 and PTK2B/PYK2. Binds ASAP2. Interacts with unphosphorylated ITGA4. Interacts with RNF5 and PDCD10. Interacts with NEK3 and this interaction is prolactin-dependent. Interacts with PTK6. Subcellular Location: Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cytoplasm, cell cortex. Note=Colocalizes with integrins at the cell periphery. Post-translational modifications: Phosphorylated by MAPK1/ERK2. Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. Phosphorylation at Ser-244 by CDK5 reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation. Phosphorylation at Tyr-31 and Tyr-118 by PTK6 promote the activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. Similarity: Belongs to the paxillin family. Contains 4 LIM zinc-binding domains. SWISS: P49023 Gene ID: 5829 Database links: Entrez Gene: 5829 Human Entrez Gene: 19303 Mouse Omim: 602505 Human SwissProt: P49023 Human SwissProt: Q8VI36 Mouse Unigene: 446336 Human Unigene: 18714 Mouse Unigene: 75 Rat Paxillin是一種分子量為68KDa的局部粘附蛋白，參與腫瘤細(xì)胞的粘附與轉(zhuǎn)移。主要用于各種惡性腫瘤的研究。 Paxillin 蛋白是胞外基質(zhì)細(xì)胞粘附（粘著斑）位點(diǎn)上肌動(dòng)蛋白-膜附著的細(xì)胞骨架蛋白。 Paxillin 在整合蛋白的信號(hào)傳導(dǎo)中起著非常重要的作用，整合蛋白介導(dǎo)的細(xì)胞骨架的再組織需要，paxillin 酪氨酸殘基的磷酸化。 Paxillin 被粘著斑激酶 (FAK)在其118位的酪氨酸殘基處磷酸化。已經(jīng)證明，paxillin 118位和31位兩個(gè)酪氨酸的磷酸化對(duì)于MM1癌細(xì)胞的遷移是重要的。Paxillin在胚胎發(fā)育、炎癥和多種腫瘤中發(fā)揮作用。 |
| 產(chǎn)品圖片 |
Sample:
MCF-7(Human) Lysate at 40 ug
NIH/3T3(Mouse)Lysate at 40 ug
Primary: Anti-Paxillin (bs-3539R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 63 kD
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Paxillin) Polyclonal Antibody, Unconjugated (bs-3539R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-Paxillin antibody (bs-3539R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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