| 產(chǎn)品編號(hào) | bs-2901R |
| 英文名稱(chēng) | Rabbit Anti-RECK antibody |
| 中文名稱(chēng) | 金屬蛋白酶抑制因子RECK抗體 |
| 別 名 | hRECK; Membrane anchored glycoprotein (metastasis and invasion); RECK protein; Reversion inducing cysteine rich protein with Kazal motifs; ST15; Suppression of tumorigenicity 15 (reversion inducing cysteine rich protein with kazal motifs); Suppressor of tumorigenicity 15; RECK_HUMAN. |
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Specific References (4) | bs-2901R has been referenced in 4 publications.
[IF=4.22] Siddesha, Jalahalli M., et al. ?Acetylsalicylic Acid Inhibits IL‐18‐Induced Cardiac Fibroblast Migration through the Induction of RECK.? Journal of cellular physiology (2013). Mouse.
[IF=3.309] Liu L et al.
Rab7 empowers renal tubular epithelial cells with autophagy-mediated protection against albumin-induced injury.Exp Cell Res. 2018 Sep 15;370(2):198-207. WB ; Rat&Human.
[IF=2.511] Zhou L et al. The role of miR-21/RECK in the inhibition of osteosarcoma by curcumin. Mol Cell Probes. 2020 Feb 17:101534. WB ; Human.
[IF=1.03] Liu, Jianfeng, et al. "RECK is involved in intimal hyperplasia in carotid arteries: A new insight in the prevention of restenosis after vascular angioplasty."Annals of Vascular Surgery (2015). WB ; Human.
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| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Rabbit,Pig,Cow,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 107kDa |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RECK: 801-900/971 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
RECK is a cysteine rich, extracellular protein with protease inhibitor like domains whose expression is suppressed strongly in many tumors and cells transformed by various kinds of oncogenes. In normal cells, this membrane anchored glycoprotein may serve as a negative regulator for matrix metalloproteinase 9, a key enzyme involved in tumor invasion and metastasis. Function: Negatively regulates matrix metalloproteinase-9 (MMP-9) by suppressing MMP-9 secretion and by direct inhibition of its enzymatic activity. RECK down-regulation by oncogenic signals may facilitate tumor invasion and metastasis. Appears to also regulate MMP-2 and MT1-MMP, which are involved in cancer progression. Subunit: Interacts with MMP-9. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Tissue Specificity: Expressed in various tissues and untransformed cells. It is undetectable in tumor-derived cell lines and oncogenically transformed cells. Post-translational modifications: N-glycosylated. Similarity: Contains 3 Kazal-like domains. SWISS: O95980 Gene ID: 313488 Database links: Entrez Gene: 313488 Human Entrez Gene: 8434 Human Omim: 605227 Human SwissProt: O95980 Human SwissProt: Q5W0K6 Human Unigene: 388918 Human Unigene: 728961 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Kidney tissue lysates
Lane 2: Mouse NIH/3T3 cell lysates
Lane 3: Human Huvec cell lysates
Lane 4: Human U87MG cell lysates
Primary: Anti-RECK (bs-2901R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 107 kDa
Observed band size: 120 kDa
Blank control:U937.
Primary Antibody (green line): Rabbit Anti-RECK antibody (bs-2901R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells wereincubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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