| 產(chǎn)品編號(hào) | bs-2803R |
| 英文名稱 | Rabbit Anti-TNFAIP3 antibody |
| 中文名稱 | 腫瘤壞死因子α誘導(dǎo)蛋白3抗體 |
| 別 名 | Zinc finger protein A20; A20; TNFA1P2; TNFAIP 3; Tnfip 3; Tnfip3; Tumor necrosis factor alpha induced protein 3; Tumor necrosis factor induced protein 3; Tumor necrosis factor inducible protein A20; MGC104522; MGC138687; MGC138688; OTU domain containing protein 7C; OTUD7C; Putative DNA binding protein A20. |
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Specific References (1) | bs-2803R has been referenced in 1 publications.
[IF=4.741] Riffo-Vasquez Y et al. Modulation of allergic inflammation in the lung by a peptide derived from mycobacteria tuberculosis chaperonin 60.1. Clin Exp Allergy. 2019 Dec 16. IHC ; Mouse.
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| 研究領(lǐng)域 | 腫瘤 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Pig,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 90kDa |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TNFAIP3: 51-150/790 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
A20 is a Cys2/Cys2 zinc finger protein that is induced by a variety of inflammatory stimuli and regulates gene expression. Specifically, A20 is induced by tumor necrosis factor (TNF) and interleukin 1 (IL-1), and acts as a negative regulator of nuclear factor κ B (NFκB) gene expression. By inhibiting NFκB activation, A20 plays a critical role in terminating NFκB responses to various stimuli. Although the C-terminal region of A20 contains seven zinc finger domains, only four of these domains are required for in vitro inhibition of TNF-induced NFκB activation. A20 also interacts with several other proteins, such as TRAF2, TRAF6 and IkB kinase (IKK) γ protein, and can thereby inhibit cell death. TXBP151, a novel A20-binding protein, may mediate the anti-apoptotic activity of A20. Involved in the negative feedback regulation of signal transduction, A20 and A20-binding proteins may be useful as novel therapeutic tools in the treatment of a variety of diseases Function: TNFAIP3 interacts with NAF1 and inhibits TNF induced NF kappa B dependent gene expression by interfering with a RIP or TRAF2 mediated transactivation signal. It inhibits programmed cell death, has a role in the function of the lymphoid system, and may contribute to the in vivo effects of TNF. Subcellular Location: Cytoplasm. Nucleus. Similarity: Belongs to the peptidase C64 family. Contains 7 A20-type zinc fingers. Contains 1 OTU domain. SWISS: P21580 Gene ID: 7128 Database links: Entrez Gene: 7128 Human Entrez Gene: 21929 Mouse Omim: 191163 Human SwissProt: P21580 Human SwissProt: Q60769 Mouse Unigene: 211600 Human Unigene: 116683 Mouse TNFAIP3是一種泛素修飾酶，是機(jī)體重要的炎癥反應(yīng)負(fù)性調(diào)節(jié)因子,也是NF-κB的負(fù)調(diào)節(jié)蛋白。TNFAIP3蛋白與很多變態(tài)反應(yīng)疾病的病理生理演變相關(guān)聯(lián)，如：包括類風(fēng)濕關(guān)節(jié)炎、冠狀動(dòng)脈疾病、銀屑病、腹腔疾病、I型糖尿病、炎癥性腸炎和系統(tǒng)性紅斑狼瘡等。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TNFAIP3) Polyclonal Antibody, Unconjugated (bs-2803R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TNFAIP3) Polyclonal Antibody, Unconjugated (bs-2803R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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