| 產(chǎn)品編號(hào) | bs-2794R |
| 英文名稱 | Rabbit Anti-RGS5 antibody |
| 中文名稱 | G蛋白信號(hào)轉(zhuǎn)導(dǎo)調(diào)節(jié)因子5抗體 |
| 別 名 | MST092; MST106; MST129; MSTP032; MSTP092; MSTP106; MSTP129; Regulator of G Protein Signalling 5; RGS 5; RGS5_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Rabbit,Pig,Dog) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 22kDa |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse RGS5: 81-181/181 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Regulator of G protein signaling (RGS) proteins are regulatory and structural components of G protein-coupled receptor complexes. RGS proteins are GTPase-activating proteins for Gi and Gq class G-alpha proteins. They accelerate transit through the cycle of GTP binding and hydrolysis and thereby accelerate signaling kinetics and termination. RGS5 seems to be an important signalling regulator in the vascular system. Present data suggest that RGS5 may be involved in the regulation of capillary growth, angiogenesis, and in the pathophysiology of stroke. Function: Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha. Subcellular Location: Isoform 1: Cytoplasm，Membrane. Isoform 2: Cytoplasm. Similarity: Contains 1 RGS domain. SWISS: O08850 Gene ID: 19737 Database links: Entrez Gene: 8490 Human Entrez Gene: 19737 Mouse Omim: 603276 Human SwissProt: O15539 Human SwissProt: O08850 Mouse Unigene: 24950 Human Unigene: 20954 Mouse Unigene: 1150 Rat RGS5是調(diào)控G-蛋白活性的信號(hào)蛋白（RGS）家族的一員，是腫瘤血管形成的一個(gè)重要調(diào)控因子.主要與G蛋白α亞基結(jié)合，發(fā)揮GTP酶激活蛋白（GAPs）作用，負(fù)性調(diào)節(jié)G蛋白信號(hào)傳導(dǎo)途徑。Rgs5在體內(nèi)多個(gè)器官都有表達(dá)，主要表達(dá)于這些組織器官的血管周細(xì)胞。 近期發(fā)現(xiàn)，Rgs5與血管的生成、發(fā)展及成熟有密切關(guān)系，影響全身各系統(tǒng)功能。同時(shí)在一些腫瘤組織和細(xì)胞中也有高表達(dá)，且與腫瘤血管的異常有關(guān)。Rgs5基因的缺失可以使得腫瘤血管正?；?，并促進(jìn)免疫治療的效果，提示其可能成為抗腫瘤血管治療的新靶點(diǎn)。另外RGS5也可能是一種腫瘤相關(guān)抗原．對(duì)腫瘤診斷及治療有著積極的意義． |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RGS5) Polyclonal Antibody, Unconjugated (bs-2794R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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