| 產(chǎn)品編號 | bs-2622R |
| 英文名稱 | Rabbit Anti-IL21R antibody |
| 中文名稱 | 白介素21受體抗體 |
| 別 名 | IL 21R; IL-21R; IL21 Receptor; IL21R; Interleukin 21 receptor; MGC10967; NILR; IL21R_HUMAN; CD360; IL-21 receptor; IL21 Receptor; Interleukin-21 receptor; Novel interleukin receptor. |
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Specific References (1) | bs-2622R has been referenced in 1 publications.
[IF=3.06] Guan, Li-juan, et al. "Increased IL-21/IL-21R expression and its proinflammatory effects in autoimmune thyroid disease." Cytokine 72.2 (2015): 160-165. IHC-P ; Human.
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| 研究領(lǐng)域 | 免疫學(xué) 細(xì)胞膜受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Cow) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 57kDa |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IL-21R: 441-538/538 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a cytokine receptor for interleukin 21 (IL21). It belongs to the type I cytokine receptors, and has been shown to form a heterodimeric receptor complex with the common gamma-chain, a receptor subunit also shared by the receptors for interleukin 2, 4, 7, 9, and 15. This receptor transduces the growth promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells. The ligand binding of this receptor leads to the activation of multiple downstream signaling molecules, including JAK1, JAK3, STAT1, and STAT3. Knockout studies of a similar gene in mouse suggest a role for this gene in regulating immunoglobulin production. Three alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2010] Function: This is a receptor for interleukin-21. Subunit: Heterodimer with the common gamma subunit. Associates with JAK1. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Selectively expressed in lymphoid tissues. Most highly expressed in thymus and spleen. Post-translational modifications: C-mannosylated at Trp-214 in the WSXWS motif, the sugar chain makes extensive hydrogen bonds with Asn-73 sugar, and bridges the two fibronectin domains transforming the V-shaped receptor into an A-frame. Similarity: Belongs to the type I cytokine receptor family. Type 4 subfamily. Contains 2 fibronectin type-III domains. SWISS: Q9HBE5 Gene ID: 50615 Database links: Entrez Gene: 50615 Human Entrez Gene: 60504 Mouse Omim: 605383 Human SwissProt: Q9HBE5 Human SwissProt: Q9JHX3 Mouse Unigene: 210546 Human Unigene: 155643 Mouse |
| 產(chǎn)品圖片 |
Sample:
Thymus (Mouse) Lysate at 40 ug
Primary: Anti- IL21R (bs-2622R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Sample:
Lymph node (Mouse) Lysate at 40 ug
Primary: Anti- IL21R (bs-2622R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Tissue/cell:mouse lymphoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IL21R Polyclonal Antibody, Unconjugated(bs-2622R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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