| 產品編號 | bs-1968R |
| 英文名稱 | Rabbit Anti-KLK14 antibody |
| 中文名稱 | 激肽釋放酶14抗體 |
| 別 名 | Kallikrein 14; kallikrein-related peptidase 14; hK14; Kallikrein-like protein 6; KLK-L6; KLKL6; GK14; MGC157618. |
| 研究領域 | 腫瘤 細胞生物 信號轉導 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rat (predicted: Human,Mouse,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細胞定位 | 細胞外基質 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KLK14: 201-267/267 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The human tissue Kallikrein gene family encodes 15 serine proteases. All Kallikreins share structural similarities including cysteine residues, a catalytic triad of His, Asp and Ser residues, typically five coding exons, and varied intron phases. Kallikreins are predominantly secreted as inactive zymogens prior to activation by cleavage of an N-terminal peptide and all function extracellularly. Kallikreins can be activated autocatalytically, via other Kallikreins, or additional proteases. While structurally similar, Kallikrein family members have distinct functions and have key roles in many physiological and pathological processes. Many human tissue Kallikreins also show promise as cancer biomarkers, which may facilitate earlier detection and characterization of many forms of cancer. Kallikrein 14, also known as kallikrein-like6 (KLK-L6), is a trypsin-like serine proteinase. Kallikrein 14 was discovered by cloning the kallikrein locus on chromosome 19 and was found to be similar to the other kallikrein family genes. Kallikrein 14 is produced by a number of tissues, including the brain, spinal cord, esophagus, salivary gland, breast, prostate, uterus, lung, thymus and thyroid. Like many of the kallikreins, Kallikrein 14 is also found in biological fluids like serum, breast milk, amniotic fluid, seminal plasma and cerebrospinal fluid. Kallikrein 14 message has been reported to be decreased in some breast cancers and in ovarian cancers, similar to Kallikrein 10. Some cell lines express Kallikrein 14 in culture and like many of the kallikreins, the production is stimulated by hormones. Recombinant kallikrein 14 was profiled on peptide substrates and found to possess both trypsin-like and chymotrypsin-like activity. The ECM proteins lamanin and collagen-IV are thought to be substrates of Kallikrein 14. Endogenous inhibitors include kallistatin, protein-C inhibitor and alpha1-proteinase inhibitor, although Kallikrein 14 can be found complexed to a number of different proteinase inhibitors. Subunit: Interacts directly with BAK1, BID, BMF and BBC3 (By similarity). Interacts directly with BCL2L11/BIM. Interacts with BAX isoform Sigma. Subcellular Location: Secreted, extracellular space. Tissue Specificity: "Highly expressed in CNS, bone marrow and fetal liver. Similarity: Belongs to the Bcl-2 family. SWISS: Q9P0G3 Gene ID: 43847 Database links: Entrez Gene: 43847 Human Entrez Gene: 317653 Mouse Omim: 606135 Human SwissProt: Q9P0G3 Human SwissProt: Q8CGR5 Mouse Unigene: 283925 Human Unigene: 247377 Mouse KLK14目前認為在宮頸鱗癌和腺癌中都有表達,是很有意義的標志物。激肽釋放酶KLK是激肽系統(tǒng)的主要限速酶，它是一組存在于多數(shù)組織和體液中的絲氨酸蛋白酶，是一種肽鏈內切酶。KLK又稱血管舒緩素，包括15個家族成員。在不同的組織中廣泛表達,具有蛋白水解酶的活性。它特異性的在碳末端切割底物肽，可裂解激肽原釋放具有活性的激肽，由激肽發(fā)揮對心血管系統(tǒng)及腎臟功能的調節(jié)作用。 |
| 產品圖片 |
Sample:Cerebrum (Rat)Lysate at 40 ug
Primary: Anti-KLK14(bs-1968R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 25'29kD
Observed band size: 29kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-KLK14 Polyclonal Antibody, Unconjugated(bs-1968R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
