| 產(chǎn)品編號 | bs-1925R |
| 英文名稱 | Rabbit Anti-UCP-1 antibody |
| 中文名稱 | 線粒體脫偶連蛋白1抗體 |
| 別 名 | Uncoupling Protein-1; UCP1; UCP 1; Mitochondrial brown fat uncoupling protein 1; mitochondrial brown fat uncoupling protein; SLC25A7; Thermogenin; UCP 1; UCP; Uncoupling protein 1; UCP1_HUMAN. |
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Specific References (13) | bs-1925R has been referenced in 13 publications.
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| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 線粒體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Rabbit,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=1μg /test,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 33kDa |
| 細胞定位 | 細胞漿 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human UCP-1: 101-200/307 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat. [provided by RefSeq]. Function: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Subunit: Acts as a dimer forming a proton channel. Subcellular Location: Mitochondrion inner membrane; Multi-pass membrane protein. Tissue Specificity: Brown adipose tissue. Similarity: Belongs to the mitochondrial carrier family. Contains 3 Solcar repeats. SWISS: P12242 Gene ID: 7350 Database links: Entrez Gene: 7350?Human Entrez Gene: 22227?Mouse Omim: 113730?Human SwissProt: P25874?Human SwissProt: P12242?Mouse Unigene: 249211?Human 線粒體及Cyto-c在細胞凋亡中的中心地位雖然在不同信號誘導(dǎo)的細胞凋亡具有一定意義，但線粒體作為細胞內(nèi)死亡信號的感受者和放大者在細胞凋亡中的重要性是勿庸置疑的。 線粒體由兩層膜包被,外膜平滑,內(nèi)膜向內(nèi)折疊形成嵴,兩層膜之間有腔,線粒體中央是基質(zhì)。基質(zhì)內(nèi)含 有與三羧酸循環(huán)所需的全部酶類,內(nèi)膜上具有呼吸鏈酶系及ATP酶復(fù)合體。線粒體能為細胞的生命活動提供場所，是細胞內(nèi)氧化磷酸化和形成ATP的主要場所。另外,線粒體有自身的DNA和遺傳體系, 但線粒體基因組的基因數(shù)量有限,因此,線粒體只是一種半自主性的細胞器。 我們每時每刻都在呼吸，目的是把氧氣吸入體內(nèi)用于制造生物體可利用的能量分子ATP。氧氣被線粒體利用制造能量的過程如同發(fā)電廠燃燒煤發(fā)電。線粒體內(nèi)有兩個主要部件參與能量的制造，一個部件叫做呼吸鏈，另一個部件叫做三磷酸腺苷酶（簡稱ATP酶）。顧名思義呼吸鏈是直接利用氧氣把食物燃燒的部件，食物中儲存有光合作用固化下來的太陽能，燃燒食物如同發(fā)電廠燃煤鍋爐的作用，目的是把固化的太陽能釋放出來推動發(fā)電機發(fā)電。ATP酶本質(zhì)上是一個可以發(fā)電的分子馬達，像鍋爐燃煤推動發(fā)電機轉(zhuǎn)動生產(chǎn)電流一樣，固化的太陽能釋放出來推動分子馬達的轉(zhuǎn)動可以制造能量分子ATP。我們每人每天大約消耗相當(dāng)于體重數(shù)量的能量分子ATP，因此，線粒體不斷制造ATP分子是維持生命活力所必需的。 線粒體是細胞有氧呼吸的主要場所.主要分為三個階段: 第一階段：在細胞質(zhì)的基質(zhì)中，一個分子的葡萄糖分解成兩個分子的丙酮酸，同時脫下4個[H]酶；在葡萄糖分解的過程中釋放出少量的能量，其中一部分能量用于合成ATP，產(chǎn)生少量的ATP。反應(yīng)式：C6H12O6酶→2丙酮酸+4[H]+少量能量 第二階段：丙酮酸進入線粒體的基質(zhì)中，兩分子丙酮酸和6個水分子中的氫全部脫下，共脫下20個[H]，丙酮被氧化分解成二氧化碳；在此過程釋放少量的能量，其中一部分用于合成ATP，產(chǎn)生少量的能量。反應(yīng)式：2丙酮酸+6H2O酶→20[H]+6CO2+少量能量 第三階段：在線粒體的內(nèi)膜上，前兩階段脫下的共24個[H]與從外界吸收或葉綠體光合作用產(chǎn)生的6個O2結(jié)合成水；在此過程中釋放大量的能量，其中一部分能量用于合成ATP，產(chǎn)生大量的能量。反應(yīng)式：24[H]+6O2酶→12H2O+大量能量。 |
| 產(chǎn)品圖片 |
Sample:
Thymus (Mouse) Lysate at 40 ug
Primary: Anti- UCP-1 (bs-1925R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD
Blank control: Mouse spleen (blue). Primary Antibody:Rabbit Anti-UCP-1 antibody(bs-1925R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Primary antibody (bs-1925R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
