| 產(chǎn)品編號 | bs-1911R |
| 英文名稱 | Rabbit Anti-TGM3 antibody |
| 中文名稱 | 谷氨酰胺轉(zhuǎn)胺酶3抗體 |
| 別 名 | Transglutaminase 3; E polypeptide protein glutamine gamma glutamyltransferase; MGC126249; MGC126250; Protein glutamine gamma glutamyltransferase E 27 kDa catalytic chain; Protein glutamine gamma glutamyltransferase E 50 kDa noncatalytic chain; Protein glutamine gamma glutamyltransferase E; TG(E); TGase E; TGE; TGM 3; TGM3; Transglutaminase E; Transglutaminase3; TransglutaminaseE; TGM3_HUMAN. |
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Specific References (1) | bs-1911R has been referenced in 1 publications.
[IF=2.47] Kawabe, Kenji, et al. "Transglutaminases Derived from Astrocytes Accelerate Amyloid β Aggregation." Neurochemical Research: 1-8. Rat.
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| 研究領(lǐng)域 | 神經(jīng)生物學 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Cow,Dog,Horse) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TGM3: 601-693/693 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium dependent. Transglutaminase 3 consists of two polypeptide chains activated from a single precursor protein by proteolysis. Transglutaminase 3 is involved the later stages of cell envelope formation in the epidermis and hair follicle. Subunit: Consists of two polypeptide chains, which are synthesized as a precursor form of a single polypeptide. Similarity: Belongs to the transglutaminase superfamily. Transglutaminase family. SWISS: Q08188 Gene ID: 7053 Database links: Entrez Gene: 7053 Human Entrez Gene: 21818 Mouse Omim: 600238 Human SwissProt: Q08188 Human SwissProt: Q08189 Mouse Unigene: 2022 Human Unigene: 2961 Mouse TGM3是轉(zhuǎn)谷氨酰胺酶家族的主要成員之一，是一種鈣離子依賴性蛋白酶，主要功能是參與角質(zhì)化細胞的終末分化過程。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TGM3) Polyclonal Antibody, Unconjugated (bs-1911R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
