| 產(chǎn)品編號 | bs-1696R |
| 英文名稱 | Rabbit Anti-DR5 antibody |
| 中文名稱 | 腫瘤細(xì)胞調(diào)亡素/死亡受體5抗體 |
| 別 名 | Tumor necrosis factor receptor superfamily member 10B; TNFRSF10B; sTRAIL Receptor 2; Death receptor 5; CD 262; CD262; CD262 antigen; Death domain containing receptor for TRAIL/Apo2L; Death receptor 5; DR 5; Fas like protein precursor; KILLER; KILLER/DR5; p53 regulated DNA damage inducible cell death receptor (killer); TNF related apoptosis inducing ligand receptor 2; TNF-related apoptosis-inducing ligand receptor 2; TRAIL R2; TRAIL receptor 2; TRAILR2; TRANCER; TRICK2; TRICK2A; TRICK2B; TRICKB; Tumor necrosis factor receptor like protein ZTNFR9; ZTNFR9; TR10B_HUMAN. |
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Specific References (3) | bs-1696R has been referenced in 3 publications.
[IF=11.556] Wen-juan Jiang. et al. Tubular epithelial cell-to-macrophage communication forms a negative feedback loop via extracellular vesicle transfer to promote renal inflammation and apoptosis in diabetic nephropathy. Theranostics. 2022; 12(1): 324–339 WB ; Mouse,Human.
[IF=7.129] Furui Han. et al. In vivo and in vitro study on hepatotoxicity of Tris-(2, 3-dibromopropyl) isocyanurate exposure via mitochondrial and death receptor pathway. ECOTOX ENVIRON SAFE. 2022 Nov;246:114186 WB ; Rat, Human.
[IF=3.057] Kong et al. Dihydroartemisinin enhances Apo2L/TRAIL-mediated apoptosis in pancreatic cancer cells via ROS-mediated up-regulation of death receptor 5. (2012) PLoS.On. 7:e37222 WB ; Human.
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| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 細(xì)胞凋亡 細(xì)胞膜受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 48kDa |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from rat DR5: 301-381/381 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. Two transcript variants encoding different isoforms and one non-coding transcript have been found for this gene. [provided by RefSeq, Mar 2009]
Function: Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis. Subcellular Location: Cell Membrane Tissue Specificity: Widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain. DISEASE: Squamous cell carcinoma of the head and neck (HNSCC) [MIM:275355]: A non-melanoma skin cancer affecting the head and neck. The hallmark of cutaneous SCC is malignant transformation of normal epidermal keratinocytes. Note=The disease may be caused by mutations affecting the gene represented in this entry. Similarity: Contains 1 death domain. Contains 3 TNFR-Cys repeats. SWISS: O14763 Gene ID: 364420 Database links: Entrez Gene: 8795 Human Entrez Gene: 21933 Mouse Omim: 603612 Human SwissProt: O14763 Human SwissProt: Q9QZM4 Mouse Unigene: 521456 Human Unigene: 193430 Mouse DR5分布比較廣泛，包括肝、脾、肺、胸腺和活化T細(xì)胞以及一些腫瘤細(xì)胞系。APO-2L在誘導(dǎo)轉(zhuǎn)化細(xì)胞和腫瘤細(xì)胞的調(diào)亡中，廣泛且迅速，多表達與胞漿。 |
| 產(chǎn)品圖片 |
Sample:
lovo Cell (Human) Lysate at 40 ug
Primary: Anti-DR5/CD262 (bs- 1696R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (DR5) polyclonal Antibody, Unconjugated (bs-1696R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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