| 產(chǎn)品編號 | bs-1691R |
| 英文名稱 | Rabbit Anti-HCN4 antibody |
| 中文名稱 | 環(huán)化核苷酸調(diào)控陽離子通道蛋白亞型4 |
| 別 名 | HCN 4;Hyperpolarization activated cyclic nucleotide gated potassium channel 4; Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 4;Hyperpolarization activated Cyclic Nucleotide-gated channel 4; HCN4_HUMAN. |
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Specific References (2) | bs-1691R has been referenced in 2 publications.
[IF=1.89] Li et al. Association between reversal in the expression of hyperpolarization-activated cyclic nucleotide-gated (HCN) channel and age-related atrial fibrillation. (2014) Med.Sci.Monit. 20:2292-7 WB ; Dog.
[IF=0] Li, Yao-Dong, et al. "Association between Reversal in the Expression of Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) Channel and Age-Related Atrial Fibrillation." American Journal of Case Reports 20 (2014): 2292-2297. WB ;
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| 研究領(lǐng)域 | 心血管 免疫學(xué) 通道蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1ug/Test,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 129kDa |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HCN4: 501-600/1203 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
HCN4 is a member of the family of hyperpolarization activated and cyclic nucleotide gated (HCN) channels. HCN currents have been linked to pacemaker activity in the heart and brain, resting potential control, as well as neuronal plasticity. It has been shown that HCN4 channels function as receptors for sour taste, and are associated with pacemaker potential generation in the sinoatrial node. Function: Hyperpolarization-activated ion channel with very slow activation and inactivation exhibiting weak selectivity for potassium over sodium ions. May contribute to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP. May mediate responses to sour stimuli. Subunit: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Highly expressed in thalamus, testis and in heart, both in ventricle and atrium. Detected at much lower levels in amygdala, substantia nigra, cerebellum and hippocampus. DISEASE: Defects in HCN4 are a cause of sick sinus syndrome type 2 (SSS2) [MIM:163800]; also known as atrial fibrillation with bradyarrhythmia or familial sinus bradycardia. The term 'sick sinus syndrome' encompasses a variety of conditions caused by sinus node dysfunction. The most common clinical manifestations are syncope, presyncope, dizziness, and fatigue. Electrocardiogram typically shows sinus bradycardia, sinus arrest, and/or sinoatrial block. Episodes of atrial tachycardias coexisting with sinus bradycardia ('tachycardia-bradycardia syndrome') are also common in this disorder. SSS occurs most often in the elderly associated with underlying heart disease or previous cardiac surgery, but can also occur in the fetus, infant, or child without heart disease or other contributing factors, in which case it is considered to be a congenital disorder. Defects in HCN4 are the cause of Brugada syndrome type 8 (BRGDA8) [MIM:613123]. A tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram (ECG). It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs (called ventricular fibrillation), the individual will faint and may die in a few minutes if the heart is not reset Similarity: Belongs to the potassium channel HCN family. Contains 1 cyclic nucleotide-binding domain. SWISS: Q9Y3Q4 Gene ID: 10021 Database links: Entrez Gene: 10021 Human Entrez Gene: 330953 Mouse Omim: 605206 Human SwissProt: Q9Y3Q4 Human SwissProt: O70507 Mouse Unigene: 86941 Human Unigene: 41082 Rat HCN4陽離子通道結(jié)構(gòu)蛋白,具有調(diào)節(jié)心臟起搏的功能蛋白。 |
| 產(chǎn)品圖片 |
Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti-HCN4 (bs-1691R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 129 kD
Observed band size: 129 kD
Sample:
Lane 1: Mouse Testis Lysates
Lane 2: Rat Testis Lysates
Lane 3: Human HL-60 cell Lysates
Lane 4: Human MCF-7 cell Lysates
Lane 5: Human A549 cell Lysates
Primary: Anti-HCN4 (bs-1691R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 129kDa
Observed band size: 129kDa
Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HCN4 Polyclonal Antibody, Unconjugated(bs-1691R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control:MCF-7.
Primary Antibody (green line): Rabbit Anti-HCN4 antibody (bs-1691R)
Dilution: 1ug/Test;
Secondary Antibody (white blue line) : Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）：Normal Rabbit IgG
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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