| 產(chǎn)品編號(hào) | bsm-1623M |
| 英文名稱 | Mouse Anti-CEA(C3) antibody |
| 中文名稱 | 癌胚抗原單克隆抗體（包被） |
| 別 名 | Carcinoembryonic antigen; Carcinoembryonic antigen related cell adhesion molecule 5; Carcinoembryonic antigen-related cell adhesion molecule 5; CD66e; CD66e antigen; CEACAM5; CEAM5_HUMAN; DKFZp781M2392; Meconium antigen 100. |
 |
Specific References (12) | bsm-1623M has been referenced in 12 publications.
|
| 研究領(lǐng)域 | 腫瘤 細(xì)胞類型標(biāo)志物 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號(hào) | C3 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CEA/CD66e/CEACAM5: full length |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M PBS (pH7.4) with 0.02% Proclin300. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM 1, CEACAM 3-CEACAM 8) and 11 pregnancy-specific glyco-protein (PSG 1-PSG 11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/ CEACAM 5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria. Function: Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins. Subunit: Homodimer. Binding of E.coli Dr adhesins leads to dissociation of the homodimer. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor Tissue Specificity: Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon. Post-translational modifications: Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate. Similarity: Belongs to the immunoglobulin superfamily. CEA family. Contains 7 Ig-like (immunoglobulin-like) domains. SWISS: P06731 Gene ID: 1048 Database links: Entrez Gene: 1048 Human Omim: 114890 Human SwissProt: P06731 Human Unigene: 709196 Human CEA是一種胚胎性抗原，主要存在于胎兒消化道上皮組織，胰腺和肝癌中。CEA廣泛存在各種上皮性腫瘤，尤其是胃腸道惡性腫瘤，CEA分布陽性類型與腫瘤的惡性度有關(guān)。CEA與EMA一樣可作為上皮性惡性腫瘤的重要標(biāo)記。 分子量為150-200 kDa。 該抗體與bsm-1624M（檢測(cè)）（Mouse Anti-Human CEA Monoclonal）配對(duì)使用；主要用于ELISA試劑盒，膠體金試劑條制備生產(chǎn)都很好。大量訂購(gòu)可來電商議。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CEA(C3)) Polyclonal Antibody, Unconjugated (bsm-1623M) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
|
