| 產(chǎn)品編號 | bs-1702R |
| 英文名稱 | Rabbit Anti-FUT4 antibody |
| 中文名稱 | α-(1,3)-巖藻糖基轉(zhuǎn)移酶 4抗體 |
| 別 名 | FUT4_HUMAN; Alpha-(1,3)-fucosyltransferase 4; 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase; EC:2.4.1.152; ELAM-1 ligand fucosyltransferase; Fucosyltransferase 4; Fucosyltransferase IV (Fuc-TIV; FucT-IV); Galactoside 3-L-fucosyltransferase; ELFT; FCT3A; LeX; CD15; FUTIV; SSEA-1; SSEA1; FUC-TIV; |
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Specific References (6) | bs-1702R has been referenced in 6 publications.
[IF=7.94] Maolin Zhang. et al. Rapid and efficient generation of cartilage pellets from mouse induced pluripotent stem cells by transcriptional activation of BMP-4 with shaking culture:. J TISSUE ENG. 2022;(): IF ; Mouse.
[IF=6.064] Xinchao Miao. et al. Epiprofin Transcriptional Activation Promotes Ameloblast Induction From Mouse Induced Pluripotent Stem Cells via the BMP-Smad Signaling Axis. FRONT BIOENG BIOTECH. 2022 Jun 21;10:890882 IF ; Mouse.
[IF=2.766] Kim et al. Klotho and S100A8/A9 as Discriminative Markers between Pre-Renal and Intrinsic Acute Kidney Injury. (2016) PLoS.One. 11:e0147255 IF ; Rat.
[IF=1.888] C Geng et al. A simple fabricated microfluidic chip for urine sample-based bladder cancer detection.(2018).J MICROMECH MICROENG. microfluidic chip ; human.
[IF=1.31] Cong, Shan, Guifang Cao, and Dongjun Liu. "Effects of different feeder layers on culture of bovine embryonic stem cell-like cells in vitro." Cytotechnology (2014): 1-11. Bovine.
[IF=0] Zschemisch, Nils-Holger, et al. "Immortalized tumor derived rat fibroblasts as feeder cells facilitate the cultivation of male embryonic stem cells from the rat strain WKY/Ztm." SpringerPlus 3.1 (2014): 588. Rat.
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| 研究領域 | 腫瘤 細胞生物 免疫學 細胞膜受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 58kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FUT4: 251-295/433 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq]. Function: May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. Subcellular Location: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi. Tissue Specificity: Highest expression in stomach and colon. It is also expressed in the lung, testis, uterus, small intestine and to a lesser extent in spleen, and ovary. Present in trace amounts in brain, thymus, heart, smooth muscle, kidney and bone marrow. Not found in liver, salivary gland and pancreas. Similarity: Belongs to the glycosyltransferase 10 family. SWISS: P22083 Gene ID: 2526 Database links: Entrez Gene: 2526 Human Omim: 104230 Human SwissProt: P22083 Human
FUT4，也稱為 ELFT 和 FCT3A，屬于糖基轉(zhuǎn)移酶 10 家族。FUT4 可催化參與 Lewis X/SSEA-1 和 VIM-2 抗原表達的 alpha-1,3 糖苷鍵。FUT4 是一種抗原表位，定義為 Lewis X 碳水化合物結(jié)構(gòu)，在鼠胚胎癌細胞 (EC)、鼠 ES 和 iPS 細胞以及鼠和人類生殖細胞上表達。它被廣泛用作小鼠未分化 ES 和 iPS 細胞的陽性表面標志物和人未分化 ES 和 iPS 細胞的陰性表面標志物。 |
| 產(chǎn)品圖片 |
Sample:A549 Cell (Human) Lysate at 40 ug Primary: Anti-FUT4(bs-1702R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58kD Observed band size: 63kD
Paraformaldehyde-fixed, paraffin embedded (Human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FUT4) Polyclonal Antibody, Unconjugated (bs-1702R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Overlay histogram showing HL 60 cells stained with bs-1702R (Green line). The cells were fixed with 90% methanol (5 min) and then permeabilized with 0.01M PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (bs-1702R,1μg/1x10^6 cells) for 30 min at 22℃. The secondary antibody used was fluorescein isothiocyanate goat anti-rabbit IgG (H+L) (bs- 0295G-FITC , Brillant blue line) at 1/200 dilution for 30 min at 22℃. Isotype control antibody was rabbit IgG (polyclonal,bs-0295P,Orange line) (1μg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of 20,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Blank control:HL-60.
Primary Antibody (green line): Rabbit Anti-FUT4 (bs-1702R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-FUT4/FITC Conjugated antibody (bs-1702R-FITC)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-FITC .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
